BMRB Entry 52607

Name: Chemical shift assignments and dynamics (hetNOE, T2) of the Q-rich N-terminus of Drosophila Ago2
Published: 2025-07-01

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52607

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignments and dynamics (hetNOE, T2) of the Q-rich N-terminus of Drosophila Ago2

Deposition date:

2024-09-11

Original release date:

2025-07-01

Authors:

Hipp, Clara; Sattler, Michael

Citation:

Citation: Hipp, Clara; Mussgnug, Selina; Kang, Hyun-Seo; Asami, Sam; Sastre, Judit; Donau, Carsten; Gemmecker, Gerd; Boekhoven, Job; Forstemann, Klaus; Sattler, Michael. "Molecular mechanisms of biomolecular condensate formation in Drosophila melanogaster siRNA biogenesis" .

Assembly members:

Assembly members:
entity_1, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGKKDKNKKGGQDSAAAPQP QQQQKQQQQRQQQPQQLQQP QQLQQPQQQQQQQPHQQQQQ SSRQQPSTSSGGSRASGFQQ GGQQQKSQDAEGWTAQKKQG KQQVQGWTKQGQQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
heteronucl_NOEs
- heteronucl_NOEs_1
heteronucl_T2_relaxation
- heteronucl_T2_relaxation_1

Data type	Count
13C chemical shifts	182
15N chemical shifts	70
1H chemical shifts	71
T2 relaxation values	66
heteronuclear NOE values	69

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ago2_Nterm	1

Entities:

Entity 1, Ago2_Nterm 113 residues - Formula weight is not available

1

MET

GLY

LYS

ASP

LYS

ASN

LYS

GLY

2

GLY

GLN

ASP

SER

ALA

PRO

GLN

PRO

3

GLN

LYS

GLN

ARG

4

GLN

PRO

GLN

LEU

GLN

PRO

5

GLN

LEU

GLN

PRO

GLN

6

GLN

PRO

HIS

GLN

7

SER

ARG

GLN

PRO

SER

THR

SER

8

GLY

SER

ARG

ALA

SER

GLY

PHE

GLN

9

GLY

GLN

LYS

SER

GLN

ASP

ALA

10

GLU

GLY

TRP

THR

ALA

GLN

LYS

GLN

GLY

11

LYS

GLN

VAL

GLN

GLY

TRP

THR

LYS

GLN

12

GLY

GLN

Samples:

sample_1: Ago2_Nterm, [U-13C; U-15N], 750 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_2: Ago2_Nterm, [U-15N], 450 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
1H-15N heteronoe	sample_2	isotropic	sample_conditions_1
T2/R2 relaxation	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5, 3.7 - collection, processing

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment, data analysis

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE III 500 MHz
Bruker AVANCE III 950 MHz