BMRB Entry 52605

Name: Backbone assignment of Loqs-PD_Nterm
Published: 2025-07-01

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52605

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of Loqs-PD_Nterm

Deposition date:

2024-09-11

Original release date:

2025-07-01

Authors:

Hipp, Clara; Sattler, Michael

Citation:

Citation: Hipp, Clara; Mussgnug, Selina; Kang, Hyun-Seo; Asami, Sam; Sastre, Judit; Donau, Carsten; Gemmecker, Gerd; Boekhoven, Job; Forstemann, Klaus; Sattler, Michael. "Molecular mechanisms of biomolecular condensate formation in Drosophila melanogaster siRNA biogenesis" .

Assembly members:

Assembly members:
entity_1, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDQENFHGSSLPQQLQNLHI QPQQASPNPVQTGFAPRRHY NNLVGLGNGNAVSGSPVKGA PLGQRHVKLKKEKISAQVAQ LSQPGQLQLSDVGDPALAGG SGLQGGVGLMGVILPSDEAL KFVSETDA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	247
15N chemical shifts	116
1H chemical shifts	116

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Loqs-PD_Nterm	1

Entities:

Entity 1, Loqs-PD_Nterm 128 residues - Formula weight is not available

1

MET

ASP

GLN

GLU

ASN

PHE

HIS

GLY

SER

2

LEU

PRO

GLN

LEU

GLN

ASN

LEU

HIS

ILE

3

GLN

PRO

GLN

ALA

SER

PRO

ASN

PRO

VAL

4

GLN

THR

GLY

PHE

ALA

PRO

ARG

HIS

TYR

5

ASN

LEU

VAL

GLY

LEU

GLY

ASN

GLY

ASN

6

ALA

VAL

SER

GLY

SER

PRO

VAL

LYS

GLY

ALA

7

PRO

LEU

GLY

GLN

ARG

HIS

VAL

LYS

LEU

LYS

8

LYS

GLU

LYS

ILE

SER

ALA

GLN

VAL

ALA

GLN

9

LEU

SER

GLN

PRO

GLY

GLN

LEU

GLN

LEU

SER

10

ASP

VAL

GLY

ASP

PRO

ALA

LEU

ALA

GLY

11

SER

GLY

LEU

GLN

GLY

VAL

GLY

LEU

MET

12

GLY

VAL

ILE

LEU

PRO

SER

ASP

GLU

ALA

LEU

13

LYS

PHE

VAL

SER

GLU

THR

ASP

ALA

Samples:

sample_1: Loqs-PD_Nterm, [U-13C; U-15N], 900 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5, 3.7 - collection, processing

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 900 MHz