BMRB Entry 52590

Name: Deciphering the RNA recognition by Musashi-1 to design protein and RNA mutants for in vitro and in vivo applications
Published: 2025-09-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52590

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Deciphering the RNA recognition by Musashi-1 to design protein and RNA mutants for in vitro and in vivo applications

Deposition date:

2024-08-23

Original release date:

2025-09-09

Authors:

Perez-Rafols, Anna; Perez-Ropero, Guillermo; Cerofolini, Linda; Sperotto, Luca; Roca-Martinez, Joel; Higuera-Rodriguez, Rosa Anahi; Russomanno, Pasquale; Buijs, Jos; Kaiser, Wolfgang; Vranken, Wim; Sattler, Michael; Danielson, Helena; Martelli, Tommaso; Provenzani, Alessandro; Fragai, Marco

Citation:

Citation: Perez-Rafols, Anna; Perez-Ropero, Guillermo; Cerofolini, Linda; Sperotto, Luca; Roca-Martinez, Joel; Higuera-Rodriguez, Rosa Anahi; Russomanno, Pasquale; Kaiser, Wolfgang; Vranken, Wim; Danielson, Helena; Provenzani, Alessandro; Martelli, Tommaso; Sattler, Michael; Buijs, Jos; Fragai, Marco. "Deciphering the RNA recognition by Musashi-1 to design protein and RNA variants for in vitro and in vivo applications" Nucleic Acids Res. 53, gkaf741-gkaf741 (2025).
PubMed: 40795964

Assembly members:

Assembly members:
entity_1, polymer, 200 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: METDAPQPGLASPDSPHDPC KMFIGGLSWQTTQEGLREYF GQFGEVKECLVMRDPLTKRS RGFGFVTFMDQAGVDKVLAQ SRHELDSKTIDPKVAFPRRA QPKMVTRTKKIFVGGLSVNT TVEDVKQYFEQFGKVDDAML MFDKTTNRHRGFGFVTFESE DIVEKVCEIHFHEINNKMVE CKKAQPKEVMSPTGSARGRS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	550
15N chemical shifts	185
1H chemical shifts	185

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MSI-1 RRM1-RRM2 tandem domain	1

Entities:

Entity 1, MSI-1 RRM1-RRM2 tandem domain 200 residues - Formula weight is not available

1	MET	GLU	THR	ASP	ALA	PRO	GLN	PRO	GLY	LEU
2	ALA	SER	PRO	ASP	SER	PRO	HIS	ASP	PRO	CYS
3	LYS	MET	PHE	ILE	GLY	GLY	LEU	SER	TRP	GLN
4	THR	THR	GLN	GLU	GLY	LEU	ARG	GLU	TYR	PHE
5	GLY	GLN	PHE	GLY	GLU	VAL	LYS	GLU	CYS	LEU
6	VAL	MET	ARG	ASP	PRO	LEU	THR	LYS	ARG	SER
7	ARG	GLY	PHE	GLY	PHE	VAL	THR	PHE	MET	ASP
8	GLN	ALA	GLY	VAL	ASP	LYS	VAL	LEU	ALA	GLN
9	SER	ARG	HIS	GLU	LEU	ASP	SER	LYS	THR	ILE
10	ASP	PRO	LYS	VAL	ALA	PHE	PRO	ARG	ARG	ALA
11	GLN	PRO	LYS	MET	VAL	THR	ARG	THR	LYS	LYS
12	ILE	PHE	VAL	GLY	GLY	LEU	SER	VAL	ASN	THR
13	THR	VAL	GLU	ASP	VAL	LYS	GLN	TYR	PHE	GLU
14	GLN	PHE	GLY	LYS	VAL	ASP	ASP	ALA	MET	LEU
15	MET	PHE	ASP	LYS	THR	THR	ASN	ARG	HIS	ARG
16	GLY	PHE	GLY	PHE	VAL	THR	PHE	GLU	SER	GLU
17	ASP	ILE	VAL	GLU	LYS	VAL	CYS	GLU	ILE	HIS
18	PHE	HIS	GLU	ILE	ASN	ASN	LYS	MET	VAL	GLU
19	CYS	LYS	LYS	ALA	GLN	PRO	LYS	GLU	VAL	MET
20	SER	PRO	THR	GLY	SER	ALA	ARG	GLY	ARG	SER

Samples:

sample_1: MSI-1 RRM1-RRM2 tandem domain, [U-100% 13C; U-100% 15N], 0.300 mM; MES 20 mM; sodium chloride 100 mM; DTT 1 mM; protease inhibitors 1 mM

sample_conditions_1: ionic strength: 0.242 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection, processing

CARA - chemical shift assignment, data analysis

NMR spectrometers:

Bruker AVANCE NEO 1200 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	GLU	THR	ASP	ALA	PRO	GLN	PRO	GLY	LEU
2	ALA	SER	PRO	ASP	SER	PRO	HIS	ASP	PRO	CYS
3	LYS	MET	PHE	ILE	GLY	GLY	LEU	SER	TRP	GLN
4	THR	THR	GLN	GLU	GLY	LEU	ARG	GLU	TYR	PHE
5	GLY	GLN	PHE	GLY	GLU	VAL	LYS	GLU	CYS	LEU
6	VAL	MET	ARG	ASP	PRO	LEU	THR	LYS	ARG	SER
7	ARG	GLY	PHE	GLY	PHE	VAL	THR	PHE	MET	ASP
8	GLN	ALA	GLY	VAL	ASP	LYS	VAL	LEU	ALA	GLN
9	SER	ARG	HIS	GLU	LEU	ASP	SER	LYS	THR	ILE
10	ASP	PRO	LYS	VAL	ALA	PHE	PRO	ARG	ARG	ALA
11	GLN	PRO	LYS	MET	VAL	THR	ARG	THR	LYS	LYS
12	ILE	PHE	VAL	GLY	GLY	LEU	SER	VAL	ASN	THR
13	THR	VAL	GLU	ASP	VAL	LYS	GLN	TYR	PHE	GLU
14	GLN	PHE	GLY	LYS	VAL	ASP	ASP	ALA	MET	LEU
15	MET	PHE	ASP	LYS	THR	THR	ASN	ARG	HIS	ARG
16	GLY	PHE	GLY	PHE	VAL	THR	PHE	GLU	SER	GLU
17	ASP	ILE	VAL	GLU	LYS	VAL	CYS	GLU	ILE	HIS
18	PHE	HIS	GLU	ILE	ASN	ASN	LYS	MET	VAL	GLU
19	CYS	LYS	LYS	ALA	GLN	PRO	LYS	GLU	VAL	MET
20	SER	PRO	THR	GLY	SER	ALA	ARG	GLY	ARG	SER

1	MET	GLU	THR	ASP	ALA	PRO	GLN	PRO	GLY	LEU
2	ALA	SER	PRO	ASP	SER	PRO	HIS	ASP	PRO	CYS
3	LYS	MET	PHE	ILE	GLY	GLY	LEU	SER	TRP	GLN
4	THR	THR	GLN	GLU	GLY	LEU	ARG	GLU	TYR	PHE
5	GLY	GLN	PHE	GLY	GLU	VAL	LYS	GLU	CYS	LEU
6	VAL	MET	ARG	ASP	PRO	LEU	THR	LYS	ARG	SER
7	ARG	GLY	PHE	GLY	PHE	VAL	THR	PHE	MET	ASP
8	GLN	ALA	GLY	VAL	ASP	LYS	VAL	LEU	ALA	GLN
9	SER	ARG	HIS	GLU	LEU	ASP	SER	LYS	THR	ILE
10	ASP	PRO	LYS	VAL	ALA	PHE	PRO	ARG	ARG	ALA
11	GLN	PRO	LYS	MET	VAL	THR	ARG	THR	LYS	LYS
12	ILE	PHE	VAL	GLY	GLY	LEU	SER	VAL	ASN	THR
13	THR	VAL	GLU	ASP	VAL	LYS	GLN	TYR	PHE	GLU
14	GLN	PHE	GLY	LYS	VAL	ASP	ASP	ALA	MET	LEU
15	MET	PHE	ASP	LYS	THR	THR	ASN	ARG	HIS	ARG
16	GLY	PHE	GLY	PHE	VAL	THR	PHE	GLU	SER	GLU
17	ASP	ILE	VAL	GLU	LYS	VAL	CYS	GLU	ILE	HIS
18	PHE	HIS	GLU	ILE	ASN	ASN	LYS	MET	VAL	GLU
19	CYS	LYS	LYS	ALA	GLN	PRO	LYS	GLU	VAL	MET
20	SER	PRO	THR	GLY	SER	ALA	ARG	GLY	ARG	SER

1	MET	GLU	THR	ASP	ALA	PRO	GLN	PRO	GLY	LEU
2	ALA	SER	PRO	ASP	SER	PRO	HIS	ASP	PRO	CYS
3	LYS	MET	PHE	ILE	GLY	GLY	LEU	SER	TRP	GLN
4	THR	THR	GLN	GLU	GLY	LEU	ARG	GLU	TYR	PHE
5	GLY	GLN	PHE	GLY	GLU	VAL	LYS	GLU	CYS	LEU
6	VAL	MET	ARG	ASP	PRO	LEU	THR	LYS	ARG	SER
7	ARG	GLY	PHE	GLY	PHE	VAL	THR	PHE	MET	ASP
8	GLN	ALA	GLY	VAL	ASP	LYS	VAL	LEU	ALA	GLN
9	SER	ARG	HIS	GLU	LEU	ASP	SER	LYS	THR	ILE
10	ASP	PRO	LYS	VAL	ALA	PHE	PRO	ARG	ARG	ALA
11	GLN	PRO	LYS	MET	VAL	THR	ARG	THR	LYS	LYS
12	ILE	PHE	VAL	GLY	GLY	LEU	SER	VAL	ASN	THR
13	THR	VAL	GLU	ASP	VAL	LYS	GLN	TYR	PHE	GLU
14	GLN	PHE	GLY	LYS	VAL	ASP	ASP	ALA	MET	LEU
15	MET	PHE	ASP	LYS	THR	THR	ASN	ARG	HIS	ARG
16	GLY	PHE	GLY	PHE	VAL	THR	PHE	GLU	SER	GLU
17	ASP	ILE	VAL	GLU	LYS	VAL	CYS	GLU	ILE	HIS
18	PHE	HIS	GLU	ILE	ASN	ASN	LYS	MET	VAL	GLU
19	CYS	LYS	LYS	ALA	GLN	PRO	LYS	GLU	VAL	MET
20	SER	PRO	THR	GLY	SER	ALA	ARG	GLY	ARG	SER