BMRB Entry 52589

Name: Backbone assignment of TMEM106B
Published: 2025-09-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52589

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of TMEM106B

Deposition date:

2024-08-20

Original release date:

2025-09-22

Authors:

Perez-Borrajero, Cecilia; Schweimer, Kristian; Hennig, Janosch

Citation:

Citation: Perez-Borrajero, Cecilia. "Zinc-mediated regulation of TMEM106B function in the endolysosomal pathway revealed through systematic identification of TRIM2/3 ubiquitination substrates" .

Assembly members:

Assembly members:
entity_1, polymer, 97 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGKSLSHLPLHSSKEDAY DGVTSENMRNGLVNSEVHNE DGRNGDVSQFPYVEFTGRDS VTCPTCQGTGRIPRGQENQL VALIPYSDQRLRPRRTK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	164
15N chemical shifts	87
1H chemical shifts	87

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TMEM106B	1
2	Zn ion	2

Entities:

Entity 1, TMEM106B 97 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

LYS

SER

LEU

SER

HIS

LEU

2

PRO

LEU

HIS

SER

LYS

GLU

ASP

ALA

TYR

3

ASP

GLY

VAL

THR

SER

GLU

ASN

MET

ARG

ASN

4

GLY

LEU

VAL

ASN

SER

GLU

VAL

HIS

ASN

GLU

5

ASP

GLY

ARG

ASN

GLY

ASP

VAL

SER

GLN

PHE

6

PRO

TYR

VAL

GLU

PHE

THR

GLY

ARG

ASP

SER

7

VAL

THR

CYS

PRO

THR

CYS

GLN

GLY

THR

GLY

8

ARG

ILE

PRO

ARG

GLY

GLN

GLU

ASN

GLN

LEU

9

VAL

ALA

LEU

ILE

PRO

TYR

SER

ASP

GLN

ARG

10

LEU

ARG

PRO

ARG

THR

LYS

Entity 2, Zn ion - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: TMEM106B, [U-99% 13C; U-99% 15N], 0.6 mM; sodium chloride 100 mM; sodium phosphate 20 mM; TCEP 0.5 mM; ZnCl2 0.01 mM

sample_conditions_1: ionic strength: 0.16 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

CARA - chemical shift assignment

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks