BMRB Entry 52585
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52585
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Citation: Dewing, Sophia; Phan, Tien; Kraft, Emma; Mittal, Jeetain; Showalter, Scott. "Acetylation-Dependent Compaction of the Histone H4 Tail Ensemble" J. Phys. Chem. B 128, 10636-10649 (2024).
PubMed: 39437158
Assembly members:
entity_1, polymer, 26 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET His6 TEV LIC
Entity Sequences (FASTA):
entity_1: SGRGKGGKGLGKGGAKRHRK
VLRDNW
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 98 |
| 15N chemical shifts | 25 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Histone H4 | 1 |
Entities:
Entity 1, Histone H4 26 residues - Formula weight is not available
| 1 | SER | GLY | ARG | GLY | LYS | GLY | GLY | LYS | GLY | LEU | ||||
| 2 | GLY | LYS | GLY | GLY | ALA | LYS | ARG | HIS | ARG | LYS | ||||
| 3 | VAL | LEU | ARG | ASP | ASN | TRP |
Samples:
sample_1: Histone H4 tail, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 50 mM; potassium chloride 150 mM
sample_conditions_1: ionic strength: 0.2 M; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 15N-13C CON | sample_1 | isotropic | sample_conditions_1 |
| 3D 15N-13C CCCON | sample_1 | isotropic | sample_conditions_1 |
| 3D 15N-13C HACANCACON | sample_1 | isotropic | sample_conditions_1 |
| 15N-13C HACANCANCO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.2.0 - collection, processing
NMRFAM-SPARKY v1.470 - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker AVANCE III 500 MHz