BMRB Entry 52584

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52584

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Title:
Assignments of 2H13C15N labelled full length GlpG based on 1H detected solid-state NMR experiments
Deposition date:
2024-08-14
Original release date:
2024-08-16
Authors:
Oster, Carl; Chevelkov, Veniamin; Lange, Adam
Citation:

Citation: Oster, Carl; Chevelkov, Veniamin; Lange, Adam. "Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG"  .

Assembly members:

Assembly members:
entity_1, polymer, 296 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MLMITSFANPRVAQAFVDYM ATQGVILTIQQHNQSDVWLA DESQAERVRAELARFLENPA DPRYLAASWQAGHTGSGLHY RRYPFFAALRERAGPVTWVM MIACVVVFIAMQILGDQEVM LWLAWPFDPTLKFEFWRYFT HALMHFSLMHILFNLLWWWY LGGAVEKRLGSGKLIVITLI SALLSGYVQQKFSGPWFGGL SGVVYALMGYVWLRGERDPQ SGIYLQRGLIIFALIWIVAG WFDLFGMSMANGAHIAGLAV GLAMAFVDSLNARKRK

Data sets:
Data typeCount
13C chemical shifts383
15N chemical shifts120
1H chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GlpG1

Entities:

Entity 1, GlpG 296 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METLEUMETILETHRSERPHEALAASNPRO
4   ARGVALALAGLNALAPHEVALASPTYRMET
5   ALATHRGLNGLYVALILELEUTHRILEGLN
6   GLNHISASNGLNSERASPVALTRPLEUALA
7   ASPGLUSERGLNALAGLUARGVALARGALA
8   GLULEUALAARGPHELEUGLUASNPROALA
9   ASPPROARGTYRLEUALAALASERTRPGLN
10   ALAGLYHISTHRGLYSERGLYLEUHISTYR
11   ARGARGTYRPROPHEPHEALAALALEUARG
12   GLUARGALAGLYPROVALTHRTRPVALMET
13   METILEALACYSVALVALVALPHEILEALA
14   METGLNILELEUGLYASPGLNGLUVALMET
15   LEUTRPLEUALATRPPROPHEASPPROTHR
16   LEULYSPHEGLUPHETRPARGTYRPHETHR
17   HISALALEUMETHISPHESERLEUMETHIS
18   ILELEUPHEASNLEULEUTRPTRPTRPTYR
19   LEUGLYGLYALAVALGLULYSARGLEUGLY
20   SERGLYLYSLEUILEVALILETHRLEUILE
21   SERALALEULEUSERGLYTYRVALGLNGLN
22   LYSPHESERGLYPROTRPPHEGLYGLYLEU
23   SERGLYVALVALTYRALALEUMETGLYTYR
24   VALTRPLEUARGGLYGLUARGASPPROGLN
25   SERGLYILETYRLEUGLNARGGLYLEUILE
26   ILEPHEALALEUILETRPILEVALALAGLY
27   TRPPHEASPLEUPHEGLYMETSERMETALA
28   ASNGLYALAHISILEALAGLYLEUALAVAL
29   GLYLEUALAMETALAPHEVALASPSERLEU
30   ASNALAARGLYSARGLYS

Samples:

sample_1: GlpG, [U-13C; U-15N; U-2H], mM; sodium chloride 150 mM; TRIS 50 mM; magnesium chloride 1 mM; DSS mM; e. coli total lipid extract 1/1 w/w

sample_conditions_1: pH: 7.4; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D hCANHsample_1anisotropicsample_conditions_1
3D hCONHsample_1anisotropicsample_conditions_1
4D hCOCANHsample_1anisotropicsample_conditions_1
4D hCACONHsample_1anisotropicsample_conditions_1
4D hCXCANH TOCSYsample_1anisotropicsample_conditions_1
4D hCXCAcoNH TOCSYsample_1anisotropicsample_conditions_1

Software:

CcpNMR v3 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks