BMRB Entry 52579

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52579

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Trp-cage fortified Tc5b-Exenatide chimera (Ex-4-Tc5bQR) at 310K
Deposition date:
2024-08-07
Original release date:
2024-09-16
Authors:
Horvath, Daniel
Citation:

Citation: Horvath, Daniel; Straner, Pal; Taricska, Nora; Fazekas, Zsolt; Menyhard, Dora; Perczel, Andras. "Influence of Trp-Cage on the Function and Stability of GLP-1R Agonist Exenatide Derivatives"  J. Med. Chem. 67, 16757-16772 (2024).
PubMed: 39254428

Assembly members:

Assembly members:
entity_1, polymer, 25 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: EEEAVRLYIQWLKQGGPSSG RPPPS

Data sets:
Data typeCount
1H chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ex-4-Tc5bQR monomer1

Entities:

Entity 1, Ex-4-Tc5bQR monomer 25 residues - Formula weight is not available

1   GLUGLUGLUALAVALARGLEUTYRILEGLN
2   TRPLEULYSGLNGLYGLYPROSERSERGLY
3   ARGPROPROPROSER

Samples:

sample_1: Ex-4-Tc5bQR 0.65 mM; sodium azide 1%; DSS 1%

sample_conditions_1: pH: 7.14; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.1. - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III 700 MHz