Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52577
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Citation: Berardi, Andrea; Quilici, Giacomo; Spiliotopoulos, Dimitrios; Corral Rodriguez, Maria Angeles; Martin Garcia, Fernando; Degano, Massimo; Tonon, Giovanni; Ghitti, Michela; Musco, Giovanna. "Structural basis for PHDVC5HCHNSD1-C2HRNizp1 interaction: implications for Sotos syndrome" Nucleic Acids Res. 44, 3448-3463 (2016).
PubMed: 26896805
Assembly members:
entity_1, polymer, 94 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet-M11
Entity Sequences (FASTA):
entity_1: GAMEREDECFSCGDAGQLVS
CKKPGCPKVYHADCLNLTKR
PAGKWECPWHQCDVCGKEAA
SFCEMCPSSFCKQHREGMLF
ISKLDGRLSCTEHD
Data type | Count |
T1 relaxation values | 79 |
T2 relaxation values | 79 |
heteronuclear NOE values | 80 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | PHDCv5HCH domain of NSD1 | 1 |
2 | ZINC ION, 1 | 2 |
3 | ZINC ION, 2 | 2 |
4 | ZINC ION, 3 | 2 |
5 | ZINC ION, 4 | 2 |
Entity 1, PHDCv5HCH domain of NSD1 94 residues - Formula weight is not available
The first three residues do not belong to the natural sequence
1 | GLY | ALA | MET | GLU | ARG | GLU | ASP | GLU | CYS | PHE | ||||
2 | SER | CYS | GLY | ASP | ALA | GLY | GLN | LEU | VAL | SER | ||||
3 | CYS | LYS | LYS | PRO | GLY | CYS | PRO | LYS | VAL | TYR | ||||
4 | HIS | ALA | ASP | CYS | LEU | ASN | LEU | THR | LYS | ARG | ||||
5 | PRO | ALA | GLY | LYS | TRP | GLU | CYS | PRO | TRP | HIS | ||||
6 | GLN | CYS | ASP | VAL | CYS | GLY | LYS | GLU | ALA | ALA | ||||
7 | SER | PHE | CYS | GLU | MET | CYS | PRO | SER | SER | PHE | ||||
8 | CYS | LYS | GLN | HIS | ARG | GLU | GLY | MET | LEU | PHE | ||||
9 | ILE | SER | LYS | LEU | ASP | GLY | ARG | LEU | SER | CYS | ||||
10 | THR | GLU | HIS | ASP |
Entity 2, ZINC ION, 1 - Zn - 65.409 Da.
1 | ZN |
sample_1: PHDvC5HCH of NSD1, [U-99% 15N], 0.3 mM; ZnCl2 10 mM; NaH2PO4/Na2HPO4 20 mM; NaCl 150 mM; TCEP 1 mM
sample_conditions_1: ionic strength: 170 mM; pH: 6.3; pressure: 1 atm; temperature: 295 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H-15N heteronoe | sample_1 | isotropic | sample_conditions_1 |
T1/R1 relaxation | sample_1 | isotropic | sample_conditions_1 |
T2/R2 relaxation | sample_1 | isotropic | sample_conditions_1 |
CcpNMR v2.5 - data analysis
NCBI | NM 008739.3 |