BMRB Entry 52576
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52576
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Citation: berardi, andrea; Leonie Kaestner, Charlotte; ghitti, michela; quilici, giacomo; cocomazzi, paolo; li, jianping; ballabio, federico; zucchelli, chiara; knapp, stefan; licht, jonathan; musco, giovanna. "The C-terminal PHDVC5HCH tandem domain of NSD2 is a combinatorial reader of unmodified H3K4 and tri-methylated H3K27 that regulates transcription of cell adhesion genes in multiple myeloma" Nucleic Acids Res. ., .-..
Assembly members:
entity_1, polymer, 105 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: into pETM11-SUMO3
Entity Sequences (FASTA):
entity_1: SRRAKGEGKRQSEDECFRCG
DGGQLVLCDRKFCTKAYHLS
CLGLGKRPFGKWECPWHHCD
VCGKPSTSFCHLCPNSFCKE
HQDGTAFSCTPDGRSYCSEH
DLGAA
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| T1 relaxation values | 90 |
| T2 relaxation values | 90 |
| heteronuclear NOE values | 94 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PHDC5HCH domain of NSD2 | 1 |
| 2 | ZINC ION, 1 | 2 |
| 3 | ZINC ION, 2 | 2 |
| 4 | ZINC ION, 3 | 2 |
| 5 | ZINC ION, 4 | 2 |
Entities:
Entity 1, PHDC5HCH domain of NSD2 105 residues - Formula weight is not available
C198 of NSD2 (C1324) (a non-Zn binding Cysteine) was mutated into Serine (i.e. C98S, C1324S NSD2) to improve solubility
| 1 | SER | ARG | ARG | ALA | LYS | GLY | GLU | GLY | LYS | ARG | ||||
| 2 | GLN | SER | GLU | ASP | GLU | CYS | PHE | ARG | CYS | GLY | ||||
| 3 | ASP | GLY | GLY | GLN | LEU | VAL | LEU | CYS | ASP | ARG | ||||
| 4 | LYS | PHE | CYS | THR | LYS | ALA | TYR | HIS | LEU | SER | ||||
| 5 | CYS | LEU | GLY | LEU | GLY | LYS | ARG | PRO | PHE | GLY | ||||
| 6 | LYS | TRP | GLU | CYS | PRO | TRP | HIS | HIS | CYS | ASP | ||||
| 7 | VAL | CYS | GLY | LYS | PRO | SER | THR | SER | PHE | CYS | ||||
| 8 | HIS | LEU | CYS | PRO | ASN | SER | PHE | CYS | LYS | GLU | ||||
| 9 | HIS | GLN | ASP | GLY | THR | ALA | PHE | SER | CYS | THR | ||||
| 10 | PRO | ASP | GLY | ARG | SER | TYR | CYS | SER | GLU | HIS | ||||
| 11 | ASP | LEU | GLY | ALA | ALA |
Entity 2, ZINC ION, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: PHDvC5HCH of NSD2, [U-99% 15N], 0.3 mM; ZnCl2 10 mM; NaH2PO4/Na2HPO4 20 mM; NaCl 150 mM; TCEP 1 mM
sample_conditions_1: ionic strength: 170 mM; pH: 6.3; pressure: 1 atm; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N heteronoe | sample_1 | isotropic | sample_conditions_1 |
| T1/R1 relaxation | sample_1 | isotropic | sample_conditions_1 |
| T2/R2 relaxation | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.5 - data analysis
NMR spectrometers:
- Bruker AVANCE III 600 MHz