BMRB Entry 52564

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52564

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Title:
1H, 13C and 15N backbone resonance assignment of the calcium-activated EndoU endoribonuclease
Deposition date:
2024-07-23
Original release date:
2024-08-28
Authors:
Malard, Florian; Campagne, Sebastien
Citation:

Citation: Malard, Florian; Karginov, Fedor; Campagne, Sebastien. "1H, 13C and 15N backbone resonance assignment of the calcium-activated EndoU endoribonuclease"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 276 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AITKEEIQSISEKIYRADTN KAQKEDIVLNSQNCISPSET RNQVDRCPKPLFTYVNEKLF SKPTYAAFINLLNNYQRATG HGEHFSAQELAEQDAFLREI MKTAVMKELYSFLHHQNRYG SEQEFVDDLKNMWFGLYSRG NEEGDSSGFEHVFSGEVKKG KVTGFHNWIRFYLEEKEGLV DYYSHIYDGPWDSYPDVLAM QFNWDGYYKEVGSAFIGSSP EFEFALYSLCFIARPGKVCQ LSLGGYPLAVRTYTWDKSTY GNGKKYIATAYIVSST

Data sets:
Data typeCount
13C chemical shifts753
15N chemical shifts251
1H chemical shifts251

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EndoU1
2Ca ion, 12
3Ca ion, 22
4Ca ion, 32
5Ca ion, 42

Entities:

Entity 1, EndoU 276 residues - Formula weight is not available

1   ALAILETHRLYSGLUGLUILEGLNSERILE
2   SERGLULYSILETYRARGALAASPTHRASN
3   LYSALAGLNLYSGLUASPILEVALLEUASN
4   SERGLNASNCYSILESERPROSERGLUTHR
5   ARGASNGLNVALASPARGCYSPROLYSPRO
6   LEUPHETHRTYRVALASNGLULYSLEUPHE
7   SERLYSPROTHRTYRALAALAPHEILEASN
8   LEULEUASNASNTYRGLNARGALATHRGLY
9   HISGLYGLUHISPHESERALAGLNGLULEU
10   ALAGLUGLNASPALAPHELEUARGGLUILE
11   METLYSTHRALAVALMETLYSGLULEUTYR
12   SERPHELEUHISHISGLNASNARGTYRGLY
13   SERGLUGLNGLUPHEVALASPASPLEULYS
14   ASNMETTRPPHEGLYLEUTYRSERARGGLY
15   ASNGLUGLUGLYASPSERSERGLYPHEGLU
16   HISVALPHESERGLYGLUVALLYSLYSGLY
17   LYSVALTHRGLYPHEHISASNTRPILEARG
18   PHETYRLEUGLUGLULYSGLUGLYLEUVAL
19   ASPTYRTYRSERHISILETYRASPGLYPRO
20   TRPASPSERTYRPROASPVALLEUALAMET
21   GLNPHEASNTRPASPGLYTYRTYRLYSGLU
22   VALGLYSERALAPHEILEGLYSERSERPRO
23   GLUPHEGLUPHEALALEUTYRSERLEUCYS
24   PHEILEALAARGPROGLYLYSVALCYSGLN
25   LEUSERLEUGLYGLYTYRPROLEUALAVAL
26   ARGTHRTYRTHRTRPASPLYSSERTHRTYR
27   GLYASNGLYLYSLYSTYRILEALATHRALA
28   TYRILEVALSERSERTHR

Entity 2, Ca ion, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: EndoU, [U-15N; U-13C], 500 uM; TRIS 10 ± 0.5 mM; sodium chloride 50 ± 2.5 mM; TCEP 1 ± 0.05 mM; calcium chloride 20 ± 1 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-13C SOFAST-HMQCsample_1isotropicsample_conditions_1
2D 1H-15N SOFAST-HMQCsample_1isotropicsample_conditions_1
3D BEST-TROSY HNCACBsample_1isotropicsample_conditions_1
3D BEST-TROSY HNCAsample_1isotropicsample_conditions_1
3D BEST-TROSY HNCOsample_1isotropicsample_conditions_1
3D BEST-TROSY HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D BEST-TROSY HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CARA v1.9 - chemical shift assignment

TOPSPIN v4.1.4 - processing

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks