BMRB Entry 52558

Name: 1H, 15N Resonance Assignments of the Truncated Forkhead Domain of the Human Forkhead Transcription Factor FOXO4
Published: 2024-11-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52558

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N Resonance Assignments of the Truncated Forkhead Domain of the Human Forkhead Transcription Factor FOXO4

Deposition date:

2024-07-18

Original release date:

2024-11-05

Authors:

Kang, Donghoon; Yang, Min June; Park, Chin-Ju

Citation:

Citation: Kang, Donghoon; Yang, Min June; Cheong, Hae-Kap; Park, Chin-Ju. "NMR investigation of FOXO4-DNA interaction for discriminating target and non-target DNA sequences" Commun. Biol. 7, 1425-1425 (2024).
PubMed: 39487330

Assembly members:

Assembly members:
entity_1, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET HIS TEV LIC cloning vector

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKSSHHHHHHENLYFQSNAG SRRNAWGNQSYAELISQAIE SAPEKRLTLAQIYEWMVRTV PYFKDKGDSNSSAGWKNSIR HNLSLHSKFIKVHNEATGKS SWWMLNPE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	61
1H chemical shifts	61

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FOXO4 FHD_DW2	1

Entities:

Entity 1, FOXO4 FHD_DW2 108 residues - Formula weight is not available

1

MET

LYS

SER

HIS

2

GLU

ASN

LEU

TYR

PHE

GLN

SER

ASN

ALA

GLY

3

SER

ARG

ASN

ALA

TRP

GLY

ASN

GLN

SER

4

TYR

ALA

GLU

LEU

ILE

SER

GLN

ALA

ILE

GLU

5

SER

ALA

PRO

GLU

LYS

ARG

LEU

THR

LEU

ALA

6

GLN

ILE

TYR

GLU

TRP

MET

VAL

ARG

THR

VAL

7

PRO

TYR

PHE

LYS

ASP

LYS

GLY

ASP

SER

ASN

8

SER

ALA

GLY

TRP

LYS

ASN

SER

ILE

ARG

9

HIS

ASN

LEU

SER

LEU

HIS

SER

LYS

PHE

ILE

10

LYS

VAL

HIS

ASN

GLU

ALA

THR

GLY

LYS

SER

11

SER

TRP

MET

LEU

ASN

PRO

GLU

Samples:

sample_1: FOXO4 FHD_DW2, [U-100% 15N], 160 uM

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 15N	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks