BMRB Entry 52557

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52557

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
The 1H, 15N, and 13C resonance assignments of a single-domain antibody against imunnoglobulin G
Deposition date:
2024-07-16
Original release date:
2024-08-16
Authors:
Leite, Vanessa; de Andrade, Rafael; Almeida, Fabio; Nascimento, Claudia; de Araujo, Talita; Almeida, Marcius
Citation:

Citation: Leite, Vanessa; de Andrade, Rafael; Almeida, Fabio; Nascimento, Claudia; de Araujo, Talita; Almeida, Marcius. "The 1 H, 15 N, and 13 C resonance assignments of a single-domain antibody against imunnoglobulin G"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Vicugna pacos   Taxonomy ID: 30538   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Vicugna pacos

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-25b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: QVQLVESGGGLVQPGGSLRL SCAASGFTFSDTWMNWVRQA PGKGLYWISAINPDGGNTAY ADSVKGRFTISRDNAKNMVY LQMDNLRPEDTAMYYCAKGW VRLPDPDLVRGQGTQVTVSS HHHHHH

Data sets:
Data typeCount
13C chemical shifts469
15N chemical shifts123
1H chemical shifts734

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nanobody anti-IgG1

Entities:

Entity 1, Nanobody anti-IgG 126 residues - Formula weight is not available

1   GLNVALGLNLEUVALGLUSERGLYGLYGLY
2   LEUVALGLNPROGLYGLYSERLEUARGLEU
3   SERCYSALAALASERGLYPHETHRPHESER
4   ASPTHRTRPMETASNTRPVALARGGLNALA
5   PROGLYLYSGLYLEUTYRTRPILESERALA
6   ILEASNPROASPGLYGLYASNTHRALATYR
7   ALAASPSERVALLYSGLYARGPHETHRILE
8   SERARGASPASNALALYSASNMETVALTYR
9   LEUGLNMETASPASNLEUARGPROGLUASP
10   THRALAMETTYRTYRCYSALALYSGLYTRP
11   VALARGLEUPROASPPROASPLEUVALARG
12   GLYGLNGLYTHRGLNVALTHRVALSERSER
13   HISHISHISHISHISHIS

Samples:

sample_1: Nanobody, [U-13C; U-15N], 500 uM; sodium chloride 150 mM; sodium phosphate 20 mM; sodium azide 3 mM; DSS 0.2 mM; H2O 90%; D2O 10 % v/v

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.5.2 - chemical shift assignment, data analysis, peak picking

TOPSPIN v4.0.6 - collection

NMRPipe v2023.129.13.28 - processing

NMRbox v2024.26.0 - data analysis

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks