BMRB Entry 52550

Name: Human CHCHD10 N-Terminus
Published: 2024-08-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52550

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human CHCHD10 N-Terminus

Deposition date:

2024-07-15

Original release date:

2024-08-15

Authors:

Mancinelli, Chiara; Lv, Guohua; Sayles, Nicole; Huang, Yun; McAvoy, Kevin; Manfredi, Giovanni; Fujita, Hibiki; Eliezer, David

Citation:

Citation: Lv, Guohua; Sayles, Nicole; Huang, Yun; Mancinelli, Chiara; McAvoy, Kevin; Shneider, Neil; Manfredi, Giovanni; Kawamata, Hibiki; Eliezer, David. "Amyloid fibril structures link CHCHD10 and CHCHD2 to neurodegeneration" bioRxiv ., .-. (2024).
PubMed: 39091724

Assembly members:

Assembly members:
entity_1, polymer, 99 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MPRGSRSAASRPASRPAAPS AHPPAHPPPSAAAPAPAPSG QPGLMAQMATTAAGVAVGSA VGHVMGSALTGAFSGGSSEP SQPAVQQAPTPAAPQPLQM

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	230
15N chemical shifts	76
1H chemical shifts	75

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hCHCHD10_NTerm	1

Entities:

Entity 1, hCHCHD10_NTerm 99 residues - Formula weight is not available

1

MET

PRO

ARG

GLY

SER

ARG

SER

ALA

SER

2

ARG

PRO

ALA

SER

ARG

PRO

ALA

PRO

SER

3

ALA

HIS

PRO

ALA

HIS

PRO

SER

4

ALA

PRO

ALA

PRO

ALA

PRO

SER

GLY

5

GLN

PRO

GLY

LEU

MET

ALA

GLN

MET

ALA

THR

6

THR

ALA

GLY

VAL

ALA

VAL

GLY

SER

ALA

7

VAL

GLY

HIS

VAL

MET

GLY

SER

ALA

LEU

THR

8

GLY

ALA

PHE

SER

GLY

SER

GLU

PRO

9

SER

GLN

PRO

ALA

VAL

GLN

ALA

PRO

THR

10

PRO

ALA

PRO

GLN

PRO

LEU

GLN

MET

Samples:

sample_1: hCHCHD10_NTerm, [U-100% 13C; U-100% 15N], 200 uM; D2O 10%; H2O 90%; sodium chloride 100 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

SPARKY - data analysis

NMRbox - data analysis

TOPSPIN - collection

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks