BMRB Entry 52549

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52549

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Title:
Backbone NMR Resonance Assignment for the Wild Type E. coli b-clamp
Deposition date:
2024-07-14
Original release date:
2024-07-18
Authors:
Mahdi, Sam; Lim, Socheata; Beuning, Penny; Korzhnev, Dmitry
Citation:

Citation: Mahdi, Sam; Lim, Socheata; Bezsonova, Irina; Beuning, Penny; Korzhnev, Dmitry. "The backbone NMR resonance assignments of the stabilized E. coli b-clamp"  Biomol. NMR Assignments 18, 293-297 (2024).
PubMed: 39269602

Assembly members:

Assembly members:
entity_1, polymer, 367 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28B+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMKFTVEREHLLKPLQQVSG PLGGRPTLPILGNLLLQVAD GTLSLTGTDLEMEMVARVAL VQPHEPGATTVPARKFFDIC RGLPEGAEIAVQLEGERMLV RSGRSRFSLSTLPAADFPNL DDWQSEVEFTLPQATMKRLI EATQFSMAHQDVRYYLNGML FETEGEELRTVATDGHRLAV CSMPIGQSLPSHSVIVPRKG VIELMRMLDGGDNPLRVQIG SNNIRAHVGDFIFTSKLVDG RFPDYRRVLPKNPDKHLEAG CDLLKQAFARAAILSNEKFR GVRLYVSENQLKITANNPEQ EEAEEILDVTYSGAEMEIGF NVSYVLDVLNALKCENVRMM LTDSVSSVQIEDAASQSAAY VVMPMRL

Data sets:
Data typeCount
13C chemical shifts260
15N chemical shifts195
1H chemical shifts195

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Wild Type E. coli b-clamp1

Entities:

Entity 1, Wild Type E. coli b-clamp 367 residues - Formula weight is not available

1   GLYMETLYSPHETHRVALGLUARGGLUHIS
2   LEULEULYSPROLEUGLNGLNVALSERGLY
3   PROLEUGLYGLYARGPROTHRLEUPROILE
4   LEUGLYASNLEULEULEUGLNVALALAASP
5   GLYTHRLEUSERLEUTHRGLYTHRASPLEU
6   GLUMETGLUMETVALALAARGVALALALEU
7   VALGLNPROHISGLUPROGLYALATHRTHR
8   VALPROALAARGLYSPHEPHEASPILECYS
9   ARGGLYLEUPROGLUGLYALAGLUILEALA
10   VALGLNLEUGLUGLYGLUARGMETLEUVAL
11   ARGSERGLYARGSERARGPHESERLEUSER
12   THRLEUPROALAALAASPPHEPROASNLEU
13   ASPASPTRPGLNSERGLUVALGLUPHETHR
14   LEUPROGLNALATHRMETLYSARGLEUILE
15   GLUALATHRGLNPHESERMETALAHISGLN
16   ASPVALARGTYRTYRLEUASNGLYMETLEU
17   PHEGLUTHRGLUGLYGLUGLULEUARGTHR
18   VALALATHRASPGLYHISARGLEUALAVAL
19   CYSSERMETPROILEGLYGLNSERLEUPRO
20   SERHISSERVALILEVALPROARGLYSGLY
21   VALILEGLULEUMETARGMETLEUASPGLY
22   GLYASPASNPROLEUARGVALGLNILEGLY
23   SERASNASNILEARGALAHISVALGLYASP
24   PHEILEPHETHRSERLYSLEUVALASPGLY
25   ARGPHEPROASPTYRARGARGVALLEUPRO
26   LYSASNPROASPLYSHISLEUGLUALAGLY
27   CYSASPLEULEULYSGLNALAPHEALAARG
28   ALAALAILELEUSERASNGLULYSPHEARG
29   GLYVALARGLEUTYRVALSERGLUASNGLN
30   LEULYSILETHRALAASNASNPROGLUGLN
31   GLUGLUALAGLUGLUILELEUASPVALTHR
32   TYRSERGLYALAGLUMETGLUILEGLYPHE
33   ASNVALSERTYRVALLEUASPVALLEUASN
34   ALALEULYSCYSGLUASNVALARGMETMET
35   LEUTHRASPSERVALSERSERVALGLNILE
36   GLUASPALAALASERGLNSERALAALATYR
37   VALVALMETPROMETARGLEU

Samples:

sample_1: Wild Type E. coli b-clamp, [U-13C; U-15N; U-2H; 99% 1HD-Ile,Leu; 99% 1HG-Val], 1 mM; Na2HPO4 100 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D HNCO TROSYsample_1isotropicsample_conditions_1
3D HNCA TROSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESY TROSYsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY v1.47 - chemical shift assignment

NMR spectrometers:

  • Agilent VNMRS 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks