BMRB Entry 52548

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52548

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Title:
Backbone NMR Resonance Assignment for the T45R_S107R E. coli b-clamp
Deposition date:
2024-07-12
Original release date:
2024-07-18
Authors:
Mahdi, Sam; Lim, Socheata; Beuning, Penny; Korzhnev, Dmitry
Citation:

Citation: Mahdi, Sam; Lim, Socheata; Bezsonova, Irina; Beuning, Penny; Korzhnev, Dmitry. "The backbone NMR resonance assignments of the stabilized E. coli b-clamp"  Biomol. NMR Assignments 18, 293-297 (2024).
PubMed: 39269602

Assembly members:

Assembly members:
entity_1, polymer, 367 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28B+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMKFTVEREHLLKPLQQVSG PLGGRPTLPILGNLLLQVAD GTLSLRGTDLEMEMVARVAL VQPHEPGATTVPARKFFDIC RGLPEGAEIAVQLEGERMLV RSGRSRFRLSTLPAADFPNL DDWQSEVEFTLPQATMKRLI EATQFSMAHQDVRYYLNGML FETEGEELRTVATDGHRLAV CSMPIGQSLPSHSVIVPRKG VIELMRMLDGGDNPLRVQIG SNNIRAHVGDFIFTSKLVDG RFPDYRRVLPKNPDKHLEAG CDLLKQAFARAAILSNEKFR GVRLYVSENQLKITANNPEQ EEAEEILDVTYSGAEMEIGF NVSYVLDVLNALKCENVRMM LTDSVSSVQIEDAASQSAAY VVMPMRL

Data sets:
Data typeCount
13C chemical shifts717
15N chemical shifts222
1H chemical shifts222

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1T45R_S107R E. coli b-clamp1

Entities:

Entity 1, T45R_S107R E. coli b-clamp 367 residues - Formula weight is not available

1   GLYMETLYSPHETHRVALGLUARGGLUHIS
2   LEULEULYSPROLEUGLNGLNVALSERGLY
3   PROLEUGLYGLYARGPROTHRLEUPROILE
4   LEUGLYASNLEULEULEUGLNVALALAASP
5   GLYTHRLEUSERLEUARGGLYTHRASPLEU
6   GLUMETGLUMETVALALAARGVALALALEU
7   VALGLNPROHISGLUPROGLYALATHRTHR
8   VALPROALAARGLYSPHEPHEASPILECYS
9   ARGGLYLEUPROGLUGLYALAGLUILEALA
10   VALGLNLEUGLUGLYGLUARGMETLEUVAL
11   ARGSERGLYARGSERARGPHEARGLEUSER
12   THRLEUPROALAALAASPPHEPROASNLEU
13   ASPASPTRPGLNSERGLUVALGLUPHETHR
14   LEUPROGLNALATHRMETLYSARGLEUILE
15   GLUALATHRGLNPHESERMETALAHISGLN
16   ASPVALARGTYRTYRLEUASNGLYMETLEU
17   PHEGLUTHRGLUGLYGLUGLULEUARGTHR
18   VALALATHRASPGLYHISARGLEUALAVAL
19   CYSSERMETPROILEGLYGLNSERLEUPRO
20   SERHISSERVALILEVALPROARGLYSGLY
21   VALILEGLULEUMETARGMETLEUASPGLY
22   GLYASPASNPROLEUARGVALGLNILEGLY
23   SERASNASNILEARGALAHISVALGLYASP
24   PHEILEPHETHRSERLYSLEUVALASPGLY
25   ARGPHEPROASPTYRARGARGVALLEUPRO
26   LYSASNPROASPLYSHISLEUGLUALAGLY
27   CYSASPLEULEULYSGLNALAPHEALAARG
28   ALAALAILELEUSERASNGLULYSPHEARG
29   GLYVALARGLEUTYRVALSERGLUASNGLN
30   LEULYSILETHRALAASNASNPROGLUGLN
31   GLUGLUALAGLUGLUILELEUASPVALTHR
32   TYRSERGLYALAGLUMETGLUILEGLYPHE
33   ASNVALSERTYRVALLEUASPVALLEUASN
34   ALALEULYSCYSGLUASNVALARGMETMET
35   LEUTHRASPSERVALSERSERVALGLNILE
36   GLUASPALAALASERGLNSERALAALATYR
37   VALVALMETPROMETARGLEU

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks