BMRB Entry 52548

Name: Backbone NMR Resonance Assignment for the T45R_S107R E. coli b-clamp
Published: 2024-07-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52548

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone NMR Resonance Assignment for the T45R_S107R E. coli b-clamp

Deposition date:

2024-07-12

Original release date:

2024-07-18

Authors:

Mahdi, Sam; Lim, Socheata; Beuning, Penny; Korzhnev, Dmitry

Citation:

Citation: Mahdi, Sam; Lim, Socheata; Beuning, Penny; Korzhnev, Dmitry. "Backbone NMR Resonance Assignment for the Wild type and Stabilized E. coli b-clamp" Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 367 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28B+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMKFTVEREHLLKPLQQVSG PLGGRPTLPILGNLLLQVAD GTLSLRGTDLEMEMVARVAL VQPHEPGATTVPARKFFDIC RGLPEGAEIAVQLEGERMLV RSGRSRFRLSTLPAADFPNL DDWQSEVEFTLPQATMKRLI EATQFSMAHQDVRYYLNGML FETEGEELRTVATDGHRLAV CSMPIGQSLPSHSVIVPRKG VIELMRMLDGGDNPLRVQIG SNNIRAHVGDFIFTSKLVDG RFPDYRRVLPKNPDKHLEAG CDLLKQAFARAAILSNEKFR GVRLYVSENQLKITANNPEQ EEAEEILDVTYSGAEMEIGF NVSYVLDVLNALKCENVRMM LTDSVSSVQIEDAASQSAAY VVMPMRL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	717
15N chemical shifts	222
1H chemical shifts	222

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	T45R_S107R E. coli b-clamp	1

Entities:

Entity 1, T45R_S107R E. coli b-clamp 367 residues - Formula weight is not available

1

GLY

MET

LYS

PHE

THR

VAL

GLU

ARG

GLU

HIS

2

LEU

LYS

PRO

LEU

GLN

VAL

SER

GLY

3

PRO

LEU

GLY

ARG

PRO

THR

LEU

PRO

ILE

4

LEU

GLY

ASN

LEU

GLN

VAL

ALA

ASP

5

GLY

THR

LEU

SER

LEU

ARG

GLY

THR

ASP

LEU

6

GLU

MET

GLU

MET

VAL

ALA

ARG

VAL

ALA

LEU

7

VAL

GLN

PRO

HIS

GLU

PRO

GLY

ALA

THR

8

VAL

PRO

ALA

ARG

LYS

PHE

ASP

ILE

CYS

9

ARG

GLY

LEU

PRO

GLU

GLY

ALA

GLU

ILE

ALA

10

VAL

GLN

LEU

GLU

GLY

GLU

ARG

MET

LEU

VAL

11

ARG

SER

GLY

ARG

SER

ARG

PHE

ARG

LEU

SER

12

THR

LEU

PRO

ALA

ASP

PHE

PRO

ASN

LEU

13

ASP

TRP

GLN

SER

GLU

VAL

GLU

PHE

THR

14

LEU

PRO

GLN

ALA

THR

MET

LYS

ARG

LEU

ILE

15

GLU

ALA

THR

GLN

PHE

SER

MET

ALA

HIS

GLN

16

ASP

VAL

ARG

TYR

LEU

ASN

GLY

MET

LEU

17

PHE

GLU

THR

GLU

GLY

GLU

LEU

ARG

THR

18

VAL

ALA

THR

ASP

GLY

HIS

ARG

LEU

ALA

VAL

19

CYS

SER

MET

PRO

ILE

GLY

GLN

SER

LEU

PRO

20

SER

HIS

SER

VAL

ILE

VAL

PRO

ARG

LYS

GLY

21

VAL

ILE

GLU

LEU

MET

ARG

MET

LEU

ASP

GLY

22

GLY

ASP

ASN

PRO

LEU

ARG

VAL

GLN

ILE

GLY

23

SER

ASN

ILE

ARG

ALA

HIS

VAL

GLY

ASP

24

PHE

ILE

PHE

THR

SER

LYS

LEU

VAL

ASP

GLY

25

ARG

PHE

PRO

ASP

TYR

ARG

VAL

LEU

PRO

26

LYS

ASN

PRO

ASP

LYS

HIS

LEU

GLU

ALA

GLY

27

CYS

ASP

LEU

LYS

GLN

ALA

PHE

ALA

ARG

28

ALA

ILE

LEU

SER

ASN

GLU

LYS

PHE

ARG

29

GLY

VAL

ARG

LEU

TYR

VAL

SER

GLU

ASN

GLN

30

LEU

LYS

ILE

THR

ALA

ASN

PRO

GLU

GLN

31

GLU

ALA

GLU

ILE

LEU

ASP

VAL

THR

32

TYR

SER

GLY

ALA

GLU

MET

GLU

ILE

GLY

PHE

33

ASN

VAL

SER

TYR

VAL

LEU

ASP

VAL

LEU

ASN

34

ALA

LEU

LYS

CYS

GLU

ASN

VAL

ARG

MET

35

LEU

THR

ASP

SER

VAL

SER

VAL

GLN

ILE

36

GLU

ASP

ALA

SER

GLN

SER

ALA

TYR

37

VAL

MET

PRO

MET

ARG

LEU

Samples:

sample_1: T45R_S107R E. coli b-clamp, [U-13C; U-15N; U-2H], 0.6 mM; Na2HPO4 100 mM; NaCl 100 mM

sample_2: T45R_S107R E. coli b-clamp, [U-13C; U-15N; U-2H; 99% 1HD-Ile,Leu; 99% 1HG-Val], 0.5 mM; Na2HPO4 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 313 K

sample_conditions_2: ionic strength: 0 mM; pH: 7.0; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO TROSY	sample_1	isotropic	sample_conditions_1
3D HNCA TROSY	sample_1	isotropic	sample_conditions_1
3D HN(CA)CB TROSY	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO TROSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY TROSY	sample_1	isotropic	sample_conditions_1
3D 13C HCH NOESY HMQC	sample_2	isotropic	sample_conditions_2
4D 13C-1H HMQC-NOESY-HMQC	sample_2	isotropic	sample_conditions_2
4D 13CH3-15NH HMQC-NOESY-TROSY	sample_2	isotropic	sample_conditions_2

Software:

NMRFAM-SPARKY v1.47 - chemical shift assignment

NMR spectrometers:

Agilent VNMRS 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks