BMRB Entry 52542

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52542

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Title:
Backbone resonance assignemnts of HUWE1 HECT domain N-lobe
Deposition date:
2024-07-09
Original release date:
2024-07-16
Authors:
Zhang, Haoran; Li, Yan
Citation:

Citation: Zhang, Haoran; Li, Yan. "Backbone resonance assignemnts of HUWE1 HECT domain N-lobe"  .

Assembly members:

Assembly members:
entity_1, polymer, 266 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPGSDFDVKRKYFRQELERL DEGLRKEDMAVHVRRDHVFE DSYRELHRKSPEEMKNRLYI VFEGEEGQDAGGLLREWYMI ISREMFNPMYALFRTSPGDR VTYTINPSSHCNPNHLSYFK FVGRIVAKAVYDNRLLECYF TRSFYKHILGKSVRYTDMES EDYHFYQGLVYLLENDVSTL GYDLTFSTEVQEFGVCEVRD LKPNGANILVTEENKKEYVH LVCQMRMTGAIRKQLAAFLE GFYEIIPKRLISIFTEQELE LLISGL

Data typeCount
13C chemical shifts645
15N chemical shifts215
1H chemical shifts215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HUWE1 HECT domain N-lobe1

Entities:

Entity 1, HUWE1 HECT domain N-lobe 266 residues - Formula weight is not available

1   GLYPROGLYSERASPPHEASPVALLYSARG
2   LYSTYRPHEARGGLNGLULEUGLUARGLEU
3   ASPGLUGLYLEUARGLYSGLUASPMETALA
4   VALHISVALARGARGASPHISVALPHEGLU
5   ASPSERTYRARGGLULEUHISARGLYSSER
6   PROGLUGLUMETLYSASNARGLEUTYRILE
7   VALPHEGLUGLYGLUGLUGLYGLNASPALA
8   GLYGLYLEULEUARGGLUTRPTYRMETILE
9   ILESERARGGLUMETPHEASNPROMETTYR
10   ALALEUPHEARGTHRSERPROGLYASPARG
11   VALTHRTYRTHRILEASNPROSERSERHIS
12   CYSASNPROASNHISLEUSERTYRPHELYS
13   PHEVALGLYARGILEVALALALYSALAVAL
14   TYRASPASNARGLEULEUGLUCYSTYRPHE
15   THRARGSERPHETYRLYSHISILELEUGLY
16   LYSSERVALARGTYRTHRASPMETGLUSER
17   GLUASPTYRHISPHETYRGLNGLYLEUVAL
18   TYRLEULEUGLUASNASPVALSERTHRLEU
19   GLYTYRASPLEUTHRPHESERTHRGLUVAL
20   GLNGLUPHEGLYVALCYSGLUVALARGASP
21   LEULYSPROASNGLYALAASNILELEUVAL
22   THRGLUGLUASNLYSLYSGLUTYRVALHIS
23   LEUVALCYSGLNMETARGMETTHRGLYALA
24   ILEARGLYSGLNLEUALAALAPHELEUGLU
25   GLYPHETYRGLUILEILEPROLYSARGLEU
26   ILESERILEPHETHRGLUGLNGLULEUGLU
27   LEULEUILESERGLYLEU

Samples:

sample_1: HUWE1 HECT domain N-lobe, [U-13C; U-15N; U-2H], 400 uM; D2O, [U-100% 2H], 5 % v/v; sodium chloride 200 mM; TRIS 20 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 222.4 mM; pH: 8.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

VNMR - collection

NMRPipe - processing

POKY - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian VNMRS 700 MHz

Related Database Links:

Uniprot Q7Z6Z7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks