BMRB Entry 52533

Name: Backbone assignments of DnaA domain IV from Bacillus subtilis (residues 328-446)
Published: 2025-01-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52533

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments of DnaA domain IV from Bacillus subtilis (residues 328-446)

Deposition date:

2024-06-28

Original release date:

2025-01-27

Authors:

Nguyen Abrams, Alexander; Kelly, Geoff; Hubbard, Julia

Citation:

Citation: Nguyen Abrams, Alexander; Kelly, Geoff; Hubbard, Julia. "NMR assignment of the conserved bacterial DNA replication protein DnaA domain IV" Biomol. NMR Assign. 18, 315-321 (2024).
PubMed: 39365420

Assembly members:

Assembly members:
entity_1, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSUMO-YHRC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: LINKDINADLAAEALKDIIP SSKPKVITIKEIQRVVGQQF NIKLEDFKAKKRTKSVAFPR QIAMYLSREMTDSSLPKIGE EFGGRDHTTVIHAHEKISKL LADDEQLQQHVKEIKEQLK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	312
15N chemical shifts	106
1H chemical shifts	106

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DnaA domain IV	1

Entities:

Entity 1, DnaA domain IV 119 residues - Formula weight is not available

Residue 1 corresponds to L328 in UNIPROT:P05648

1

LEU

ILE

ASN

LYS

ASP

ILE

ASN

ALA

ASP

LEU

2

ALA

GLU

ALA

LEU

LYS

ASP

ILE

PRO

3

SER

LYS

PRO

LYS

VAL

ILE

THR

ILE

LYS

4

GLU

ILE

GLN

ARG

VAL

GLY

GLN

PHE

5

ASN

ILE

LYS

LEU

GLU

ASP

PHE

LYS

ALA

LYS

6

LYS

ARG

THR

LYS

SER

VAL

ALA

PHE

PRO

ARG

7

GLN

ILE

ALA

MET

TYR

LEU

SER

ARG

GLU

MET

8

THR

ASP

SER

LEU

PRO

LYS

ILE

GLY

GLU

9

GLU

PHE

GLY

ARG

ASP

HIS

THR

VAL

10

ILE

HIS

ALA

HIS

GLU

LYS

ILE

SER

LYS

LEU

11

LEU

ALA

ASP

GLU

GLN

LEU

GLN

HIS

12

VAL

LYS

GLU

ILE

LYS

GLU

GLN

LEU

LYS

Samples:

sample_1: DnaA domain IV from Bacillus subtilis (residues 328-446), [U-99% 13C; U-99% 15N], 370 ± 30 uM; NaPi 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 0.12 M; pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker AVANCE III 800 MHz