BMRB Entry 52525

Name: PHL4 Effector Domain Assignments
Published: 2024-08-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52525

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

PHL4 Effector Domain Assignments

Deposition date:

2024-06-25

Original release date:

2024-08-15

Authors:

Fonda, Blake; Murray, Dylan

Citation:

Citation: Fonda, Blake; Murray, Dylan. "The Potent PHL4 Transcription Factor Effector Domain Contains Significant Disorder" bioRxiv ., .-. (2024).
PubMed: 39005418

Assembly members:

Assembly members:
entity_1, polymer, 228 residues, 24963 Da.

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMIPNDDDDANSMKNYPLND DDANSMKNYPLNDDDANSME NYPLRSIPTELSHTCSLIPP SLPNPSEAAADMSFNSELNQ IMARPCDMLPANGGAVGHNP FLEPGFNCPETTDWIPSPLP HIYFPSGSPNLIMEDGVIDE IHKQSDLPLWYDDLITTDED PLMSSILGDLLLDTNFNSAS KVQQPSMQSQIQQPQAVLQQ PSSCVELRPLDRTVSSNSNN NSNSNNAA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	527
15N chemical shifts	154
1H chemical shifts	527

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PHL4	1

Entities:

Entity 1, PHL4 228 residues - 24963 Da.

1

GLY

MET

ILE

PRO

ASN

ASP

ALA

2

ASN

SER

MET

LYS

ASN

TYR

PRO

LEU

ASN

ASP

3

ASP

ALA

ASN

SER

MET

LYS

ASN

TYR

PRO

4

LEU

ASN

ASP

ALA

ASN

SER

MET

GLU

5

ASN

TYR

PRO

LEU

ARG

SER

ILE

PRO

THR

GLU

6

LEU

SER

HIS

THR

CYS

SER

LEU

ILE

PRO

7

SER

LEU

PRO

ASN

PRO

SER

GLU

ALA

8

ASP

MET

SER

PHE

ASN

SER

GLU

LEU

ASN

GLN

9

ILE

MET

ALA

ARG

PRO

CYS

ASP

MET

LEU

PRO

10

ALA

ASN

GLY

ALA

VAL

GLY

HIS

ASN

PRO

11

PHE

LEU

GLU

PRO

GLY

PHE

ASN

CYS

PRO

GLU

12

THR

ASP

TRP

ILE

PRO

SER

PRO

LEU

PRO

13

HIS

ILE

TYR

PHE

PRO

SER

GLY

SER

PRO

ASN

14

LEU

ILE

MET

GLU

ASP

GLY

VAL

ILE

ASP

GLU

15

ILE

HIS

LYS

GLN

SER

ASP

LEU

PRO

LEU

TRP

16

TYR

ASP

LEU

ILE

THR

ASP

GLU

ASP

17

PRO

LEU

MET

SER

ILE

LEU

GLY

ASP

LEU

18

LEU

ASP

THR

ASN

PHE

ASN

SER

ALA

SER

19

LYS

VAL

GLN

PRO

SER

MET

GLN

SER

GLN

20

ILE

GLN

PRO

GLN

ALA

VAL

LEU

GLN

21

PRO

SER

CYS

VAL

GLU

LEU

ARG

PRO

LEU

22

ASP

ARG

THR

VAL

SER

ASN

SER

ASN

23

ASN

SER

ASN

SER

ASN

ALA

Samples:

sample_1: PHL4 Effector Domain, [U-100% 13C; U-100% 15N], 73.2 uM; MES 20 mM; sodium chloride 25 mM; TCEP 5 mM

sample_2: PHL4 Effector Domain, [U-100% 15N], 98.2 uM; MES 20 mM; sodium chloride 25 mM; TCEP 5 mM

sample_conditions_1: pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHANH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v10.6 - processing

NMRFAM-SPARKY v1.470 - peak picking

NMR spectrometers:

Bruker AVANCE III 800 MHz
Bruker AVANCE III 600 MHz