BMRB Entry 52524

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52524

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Title:
PHL4 Resonance Assignments
Deposition date:
2024-06-25
Original release date:
2024-08-15
Authors:
Fonda, Blake; Murray, Dylan
Citation:

Citation: Fonda, Blake; Murray, Dylan. "The Potent PHL4 Transcription Factor Effector Domain Contains Significant Disorder"  bioRxiv ., .-. (2024).
PubMed: 39005418

Assembly members:

Assembly members:
entity_1, polymer, 398 residues, 44169 Da.

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMIPNDDDDANSMKNYPLND DDANSMKNYPLNDDDANSME NYPLRSIPTELSHTCSLIPP SLPNPSEAAADMSFNSELNQ IMARPCDMLPANGGAVGHNP FLEPGFNCPETTDWIPSPLP HIYFPSGSPNLIMEDGVIDE IHKQSDLPLWYDDLITTDED PLMSSILGDLLLDTNFNSAS KVQQPSMQSQIQQPQAVLQQ PSSCVELRPLDRTVSSNSNN NSNSNNAAAAAKGRMRWTPE LHEVFVDAVNQLGGSNEATP KGVLKHMKVEGLTIFHVKSH LQKYRTAKYIPVPSEGSPEA RLTPLEQITSDDTKRGIDIT ETLRIQMEHQKKLHEQLESL RTMQLRIEEQGKALLMMIEK QNMGFGGPEQGEKTSAKTPE NGSEESESPRPKRPRNEE

Data sets:
Data typeCount
13C chemical shifts409
15N chemical shifts116
1H chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PHL41

Entities:

Entity 1, PHL4 398 residues - 44169 Da.

1   GLYMETILEPROASNASPASPASPASPALA
2   ASNSERMETLYSASNTYRPROLEUASNASP
3   ASPASPALAASNSERMETLYSASNTYRPRO
4   LEUASNASPASPASPALAASNSERMETGLU
5   ASNTYRPROLEUARGSERILEPROTHRGLU
6   LEUSERHISTHRCYSSERLEUILEPROPRO
7   SERLEUPROASNPROSERGLUALAALAALA
8   ASPMETSERPHEASNSERGLULEUASNGLN
9   ILEMETALAARGPROCYSASPMETLEUPRO
10   ALAASNGLYGLYALAVALGLYHISASNPRO
11   PHELEUGLUPROGLYPHEASNCYSPROGLU
12   THRTHRASPTRPILEPROSERPROLEUPRO
13   HISILETYRPHEPROSERGLYSERPROASN
14   LEUILEMETGLUASPGLYVALILEASPGLU
15   ILEHISLYSGLNSERASPLEUPROLEUTRP
16   TYRASPASPLEUILETHRTHRASPGLUASP
17   PROLEUMETSERSERILELEUGLYASPLEU
18   LEULEUASPTHRASNPHEASNSERALASER
19   LYSVALGLNGLNPROSERMETGLNSERGLN
20   ILEGLNGLNPROGLNALAVALLEUGLNGLN
21   PROSERSERCYSVALGLULEUARGPROLEU
22   ASPARGTHRVALSERSERASNSERASNASN
23   ASNSERASNSERASNASNALAALAALAALA
24   ALALYSGLYARGMETARGTRPTHRPROGLU
25   LEUHISGLUVALPHEVALASPALAVALASN
26   GLNLEUGLYGLYSERASNGLUALATHRPRO
27   LYSGLYVALLEULYSHISMETLYSVALGLU
28   GLYLEUTHRILEPHEHISVALLYSSERHIS
29   LEUGLNLYSTYRARGTHRALALYSTYRILE
30   PROVALPROSERGLUGLYSERPROGLUALA
31   ARGLEUTHRPROLEUGLUGLNILETHRSER
32   ASPASPTHRLYSARGGLYILEASPILETHR
33   GLUTHRLEUARGILEGLNMETGLUHISGLN
34   LYSLYSLEUHISGLUGLNLEUGLUSERLEU
35   ARGTHRMETGLNLEUARGILEGLUGLUGLN
36   GLYLYSALALEULEUMETMETILEGLULYS
37   GLNASNMETGLYPHEGLYGLYPROGLUGLN
38   GLYGLULYSTHRSERALALYSTHRPROGLU
39   ASNGLYSERGLUGLUSERGLUSERPROARG
40   PROLYSARGPROARGASNGLUGLU

Samples:

sample_1: PHL4, [U-100% 13C; U-100% 15N], 114.9 uM; MES 20 mM; sodium chloride 25 mM; TCEP 5 mM

sample_conditions_1: pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe v10.6 - processing

NMRFAM-SPARKY v1.470 - peak picking

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Related Database Links:

UNP Q8GXC2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks