BMRB Entry 52503

Name: 1H, 13C, and 15N backbone chemical shift assignments of TauK18
Published: 2024-06-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52503

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N backbone chemical shift assignments of TauK18

Deposition date:

2024-06-14

Original release date:

2024-06-28

Authors:

Muwonge, Kevin; Volkov, Alexander; Tompa, Peter

Citation:

Citation: Muwonge, Kevin; Yaman, Bedri; Meszaros, Attila; Russo, Giorgio; Volkov, Alexander; Tompa, Peter. "Improved expression of aggregation-prone Tau proteins using a spidroin-derived solubility tag" Separations 11, 198-198 (2024).

Assembly members:

Assembly members:
entity_1, polymer, 149 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: MaSp-Tau-MTBR

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKVAVVRTPPKSPSSAKSRL QTAPVPMPDLKNVKSKIGST ENLKHQPGGGKVQIINKKLD LSNVQSKCGSKDNIKHVPGG GSVQIVYKPVDLSKVTSKCG SLGNIHHKPGGGQVEVKSEK LDFKDRVQSKIGSLDNITHV PGGGNKKIE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	390
15N chemical shifts	126
1H chemical shifts	126

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	tauK18	1

Entities:

Entity 1, tauK18 149 residues - Formula weight is not available

1

MET

LYS

VAL

ALA

VAL

ARG

THR

PRO

2

LYS

SER

PRO

SER

ALA

LYS

SER

ARG

LEU

3

GLN

THR

ALA

PRO

VAL

PRO

MET

PRO

ASP

LEU

4

LYS

ASN

VAL

LYS

SER

LYS

ILE

GLY

SER

THR

5

GLU

ASN

LEU

LYS

HIS

GLN

PRO

GLY

6

LYS

VAL

GLN

ILE

ASN

LYS

LEU

ASP

7

LEU

SER

ASN

VAL

GLN

SER

LYS

CYS

GLY

SER

8

LYS

ASP

ASN

ILE

LYS

HIS

VAL

PRO

GLY

9

GLY

SER

VAL

GLN

ILE

VAL

TYR

LYS

PRO

VAL

10

ASP

LEU

SER

LYS

VAL

THR

SER

LYS

CYS

GLY

11

SER

LEU

GLY

ASN

ILE

HIS

LYS

PRO

GLY

12

GLY

GLN

VAL

GLU

VAL

LYS

SER

GLU

LYS

13

LEU

ASP

PHE

LYS

ASP

ARG

VAL

GLN

SER

LYS

14

ILE

GLY

SER

LEU

ASP

ASN

ILE

THR

HIS

VAL

15

PRO

GLY

ASN

LYS

ILE

GLU

Samples:

sample_1: TauK18, [U-100% 13C; U-100% 15N], 0.4 mM; TRIS 20 mM; sodium chloride 100 mM; D2O, [U-100% 2H], 6%

sample_conditions_1: ionic strength: 118 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.6 - collection, processing

qMDD - processing

CcpNMR v2.4 - chemical shift assignment, data analysis, peak picking

NMRPipe - processing

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks