BMRB Entry 52501
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52501
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NMR-STAR v3 text file.
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Citation: Khuntia, Harisankar; Yu, Tsyr-Yan. "Chemical shift assignments for VDAC-2 Cys-less mutant" .
Assembly members:
entity_1, polymer, 294 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b
Entity Sequences (FASTA):
entity_1: MATHGQTSARPMAIPPSYAD
LGKAARDIFNKGFGFGLVKL
DVKTKSESGVEFSTSGSSNT
DTGKVTGTLETKYKWTEYGL
TFTAKWNTDNTLGTEIAIED
QIAQGLKLTFDTTFSPNTGK
KSGKIKSSYKREHINLGSDV
DFDFAGPAIHGSAVFGYEGW
LAGYQMTFDSAKSKLTRNNF
AVGYRTGDFQLHTNVNDGTE
FGGSIYQKVNEDLDTSVNLA
WTSGTNNTRFGIAAKYQLDP
TASISAKVNNSSLIGVGYTQ
TLRPGVKLTLSALVDGKSIN
AGGHKVGLALELEA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 273 |
| 15N chemical shifts | 273 |
| 1H chemical shifts | 273 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cys-less mutant of hVDAC-2 | 1 |
Entities:
Entity 1, Cys-less mutant of hVDAC-2 294 residues - Formula weight is not available
| 1 | MET | ALA | THR | HIS | GLY | GLN | THR | SER | ALA | ARG | ||||
| 2 | PRO | MET | ALA | ILE | PRO | PRO | SER | TYR | ALA | ASP | ||||
| 3 | LEU | GLY | LYS | ALA | ALA | ARG | ASP | ILE | PHE | ASN | ||||
| 4 | LYS | GLY | PHE | GLY | PHE | GLY | LEU | VAL | LYS | LEU | ||||
| 5 | ASP | VAL | LYS | THR | LYS | SER | GLU | SER | GLY | VAL | ||||
| 6 | GLU | PHE | SER | THR | SER | GLY | SER | SER | ASN | THR | ||||
| 7 | ASP | THR | GLY | LYS | VAL | THR | GLY | THR | LEU | GLU | ||||
| 8 | THR | LYS | TYR | LYS | TRP | THR | GLU | TYR | GLY | LEU | ||||
| 9 | THR | PHE | THR | ALA | LYS | TRP | ASN | THR | ASP | ASN | ||||
| 10 | THR | LEU | GLY | THR | GLU | ILE | ALA | ILE | GLU | ASP | ||||
| 11 | GLN | ILE | ALA | GLN | GLY | LEU | LYS | LEU | THR | PHE | ||||
| 12 | ASP | THR | THR | PHE | SER | PRO | ASN | THR | GLY | LYS | ||||
| 13 | LYS | SER | GLY | LYS | ILE | LYS | SER | SER | TYR | LYS | ||||
| 14 | ARG | GLU | HIS | ILE | ASN | LEU | GLY | SER | ASP | VAL | ||||
| 15 | ASP | PHE | ASP | PHE | ALA | GLY | PRO | ALA | ILE | HIS | ||||
| 16 | GLY | SER | ALA | VAL | PHE | GLY | TYR | GLU | GLY | TRP | ||||
| 17 | LEU | ALA | GLY | TYR | GLN | MET | THR | PHE | ASP | SER | ||||
| 18 | ALA | LYS | SER | LYS | LEU | THR | ARG | ASN | ASN | PHE | ||||
| 19 | ALA | VAL | GLY | TYR | ARG | THR | GLY | ASP | PHE | GLN | ||||
| 20 | LEU | HIS | THR | ASN | VAL | ASN | ASP | GLY | THR | GLU | ||||
| 21 | PHE | GLY | GLY | SER | ILE | TYR | GLN | LYS | VAL | ASN | ||||
| 22 | GLU | ASP | LEU | ASP | THR | SER | VAL | ASN | LEU | ALA | ||||
| 23 | TRP | THR | SER | GLY | THR | ASN | ASN | THR | ARG | PHE | ||||
| 24 | GLY | ILE | ALA | ALA | LYS | TYR | GLN | LEU | ASP | PRO | ||||
| 25 | THR | ALA | SER | ILE | SER | ALA | LYS | VAL | ASN | ASN | ||||
| 26 | SER | SER | LEU | ILE | GLY | VAL | GLY | TYR | THR | GLN | ||||
| 27 | THR | LEU | ARG | PRO | GLY | VAL | LYS | LEU | THR | LEU | ||||
| 28 | SER | ALA | LEU | VAL | ASP | GLY | LYS | SER | ILE | ASN | ||||
| 29 | ALA | GLY | GLY | HIS | LYS | VAL | GLY | LEU | ALA | LEU | ||||
| 30 | GLU | LEU | GLU | ALA |
Samples:
sample_1: hVDAC2-DeltaC, [U-99% 13C; U-99% 15N; U-99% 2H], 982 uM; NaPi 25 mM; NaCl 100 mM; TCEP 20 mM; LDAO 250 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks