BMRB Entry 5250

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5250

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Title:
1H, 13C and 15N resonance assignment of the RNA-binding domain dimer form Bacillus subtilis transcriptional antiterminator GlcT
Deposition date:
2002-01-08
Original release date:
2002-09-26
Authors:
Stoldt, Matthias; Langbein, Ines; Stuelke, Joerg; Gorlach, Matthias
Citation:

Citation: Stoldt, Matthias; Langbein, Ines; Stuelke, Joerg; Gorlach, Matthias. "1H, 13C and 15N resonance assignment of the RNA-binding domain dimer form Bacillus subtilis transcriptional antiterminator GlcT"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
RNA-binding domain of transcriptional antiterminator GlcT, polymer, 74 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA-binding domain of transcriptional antiterminator GlcT: MRGSHHHHHHGSVDVNGSFT VKKVLNNNVLIASHHKYSEV VLIGKGIGFGKKQDDVIEDK GYDKMFILKDEKEQ

Data sets:
Data typeCount
13C chemical shifts298
15N chemical shifts71
1H chemical shifts485

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GlcT-RBD subunit 11
2GlcT-RBD subunit 21

Entities:

Entity 1, GlcT-RBD subunit 1 74 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERVALASPVALASNGLYSERPHETHR
3   VALLYSLYSVALLEUASNASNASNVALLEU
4   ILEALASERHISHISLYSTYRSERGLUVAL
5   VALLEUILEGLYLYSGLYILEGLYPHEGLY
6   LYSLYSGLNASPASPVALILEGLUASPLYS
7   GLYTYRASPLYSMETPHEILELEULYSASP
8   GLULYSGLUGLN

Samples:

sample_1: RNA-binding domain of transcriptional antiterminator GlcT, [U-13C; U-15N], 2.0 – 3.0 mM

cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian UnityINOVA 600 MHz
  • Varian UnityINOVA 750 MHz

Related Database Links:

PDB
DBJ BAI84997 BAM52031 BAM57608 GAK78204
EMBL CAA72077 CAB13261 CCU57949 CEI56549 CEJ76972
GB ADM37475 ADV96407 AEP86362 AEP90533 AFI28059
REF NP_389271 WP_003218635 WP_003232429 WP_003238961 WP_009967114
SP O31691
AlphaFold O31691

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