BMRB Entry 52493

Name: Lip-tagged LRP6 1539-1613
Published: 2025-09-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52493

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Lip-tagged LRP6 1539-1613

Deposition date:

2024-06-04

Original release date:

2025-09-16

Authors:

Rutherford, Trevor; Li, Tie-Mei; Franco-Echevarria, Elsa; Gammons, Melissa; Bienz, Mariann

Citation:

Citation: Gammons, Melissa; Franco-Echevarria, Elsa; Li, Tie-Mei; Rutherford, Trevor; Renko, Miha; Batters, Christopher; Bienz, Mariann. "Wnt signalosome assembly is governed by conformational flexibility of Axin and by the AP2 clathrin adaptor" Nat. Commun. 16, 4718-4718 (2025).
PubMed: 40399324

Assembly members:

Assembly members:
entity_1, polymer, 179 residues, 19691 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MHHHHHSGAFEFKLPDIGEG IHEGEIVKWFVKPDGEVNED DVLCEVQNDKAVVEIPSPVK GKVLEILVPEGTVATVGQTL ITLDAPGYENMTTGSDTGEN LYFQSDYAPSRRMTSVATAK GYTSDLNYDSEPVPPPPTPR SQYLSAEENYESCPPSPYTE RSYSHHLYPPPPSPCTDSS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	207
15N chemical shifts	67
1H chemical shifts	118

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Lip-L2	1

Entities:

Entity 1, Lip-L2 179 residues - 19691 Da.

WT sequence LRP6 1539-1613

1

MET

HIS

SER

GLY

ALA

PHE

2

GLU

PHE

LYS

LEU

PRO

ASP

ILE

GLY

GLU

GLY

3

ILE

HIS

GLU

GLY

GLU

ILE

VAL

LYS

TRP

PHE

4

VAL

LYS

PRO

ASP

GLY

GLU

VAL

ASN

GLU

ASP

5

ASP

VAL

LEU

CYS

GLU

VAL

GLN

ASN

ASP

LYS

6

ALA

VAL

GLU

ILE

PRO

SER

PRO

VAL

LYS

7

GLY

LYS

VAL

LEU

GLU

ILE

LEU

VAL

PRO

GLU

8

GLY

THR

VAL

ALA

THR

VAL

GLY

GLN

THR

LEU

9

ILE

THR

LEU

ASP

ALA

PRO

GLY

TYR

GLU

ASN

10

MET

THR

GLY

SER

ASP

THR

GLY

GLU

ASN

11

LEU

TYR

PHE

GLN

SER

ASP

TYR

ALA

PRO

SER

12

ARG

MET

THR

SER

VAL

ALA

THR

ALA

LYS

13

GLY

TYR

THR

SER

ASP

LEU

ASN

TYR

ASP

SER

14

GLU

PRO

VAL

PRO

THR

PRO

ARG

15

SER

GLN

TYR

LEU

SER

ALA

GLU

ASN

TYR

16

GLU

SER

CYS

PRO

SER

PRO

TYR

THR

GLU

17

ARG

SER

TYR

SER

HIS

LEU

TYR

PRO

18

PRO

SER

PRO

CYS

THR

ASP

SER

Samples:

sample_1: Lip-LRP6-L1(YYYF>AAAA), [U-99% 13C; U-99% 15N], 300 uM; H2O 95%; D2O, [U-99% 2H], 5%; sodium phosphate 25 mM; sodium chloride 150 mM

sample_2: Lip-LRP6-L1(YYYF>AAAA), [U-99% 13C; U-99% 15N], 300 uM; H2O 95%; D2O, [U-99% 2H], 5%; sodium phosphate 25 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 210 mM; pH: 6.7; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
(H)CBCACON	sample_2	isotropic	sample_conditions_1
(H)CBCANCO	sample_2	isotropic	sample_conditions_1
H(CA)NCO	sample_2	isotropic	sample_conditions_1
H(CACO)NCO	sample_2	isotropic	sample_conditions_1

Software:

TOPSPIN v3 - collection, processing

NMRFAM-SPARKY v1.3 - peak picking

MARS v1.1 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE II+ 700 MHz