BMRB Entry 52483

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52483

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Title:
Lip-tagged LRP6 1463-1538 (Y1517, Y1520, Y1522, F1525) > A quadruple mutant
Deposition date:
2024-05-23
Original release date:
2025-09-16
Authors:
Rutherford, Trevor; Li, Tie-Mei; Franco-Echevarria, Elsa; Gammons, Melissa; Bienz, Mariann
Citation:

Citation: Gammons, Melissa; Franco-Echevarria, Elsa; Li, Tie-Mei; Rutherford, Trevor; Renko, Miha; Batters, Christopher; Bienz, Mariann. "Wnt signalosome assembly is governed by conformational flexibility of Axin and by the AP2 clathrin adaptor"  Nat. Commun. 16, 4718-4718 (2025).
PubMed: 40399324

Assembly members:

Assembly members:
entity_1, polymer, 180 residues, 19296 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MHHHHHSGAFEFKLPDIGEG IHEGEIVKWFVKPDGEVNED DVLCEVQNDKAVVEIPSPVK GKVLEILVPEGTVATVGQTL ITLDAPGYENMTTGSDTGEN LYFQAHVTGASSSSSSSTKG TYFPAILNPPPSPATERSHY TMEFGYSSNSPSTHRSYSAR PASARHAAPPTTPCSTDVCD

Data sets:
Data typeCount
13C chemical shifts199
15N chemical shifts68
1H chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Lip-L11

Entities:

Entity 1, Lip-L1 180 residues - 19296 Da.

Quadruple mutant; (Y1517, Y1520, Y1522, F1525) > A

1   METHISHISHISHISHISSERGLYALAPHE
2   GLUPHELYSLEUPROASPILEGLYGLUGLY
3   ILEHISGLUGLYGLUILEVALLYSTRPPHE
4   VALLYSPROASPGLYGLUVALASNGLUASP
5   ASPVALLEUCYSGLUVALGLNASNASPLYS
6   ALAVALVALGLUILEPROSERPROVALLYS
7   GLYLYSVALLEUGLUILELEUVALPROGLU
8   GLYTHRVALALATHRVALGLYGLNTHRLEU
9   ILETHRLEUASPALAPROGLYTYRGLUASN
10   METTHRTHRGLYSERASPTHRGLYGLUASN
11   LEUTYRPHEGLNALAHISVALTHRGLYALA
12   SERSERSERSERSERSERSERTHRLYSGLY
13   THRTYRPHEPROALAILELEUASNPROPRO
14   PROSERPROALATHRGLUARGSERHISTYR
15   THRMETGLUPHEGLYTYRSERSERASNSER
16   PROSERTHRHISARGSERTYRSERALAARG
17   PROALASERALAARGHISALAALAPROPRO
18   THRTHRPROCYSSERTHRASPVALCYSASP

Samples:

sample_1: Lip-LRP6-L1(YYYF>AAAA), [U-99% 13C; U-99% 15N], 500 uM; H2O 95%; D2O, [U-99% 2H], 5%; sodium phosphate 25 mM; sodium chloride 150 mM

sample_2: Lip-LRP6-L1(YYYF>AAAA), [U-99% 13C; U-99% 15N], 300 uM; H2O 95%; D2O, [U-99% 2H], 5%; sodium phosphate 25 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 210 mM; pH: 6.7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
HACANsample_1isotropicsample_conditions_1
(H)CBCACONsample_1isotropicsample_conditions_1
(H)CBCANCOsample_1isotropicsample_conditions_1
H(CA)NCOsample_2isotropicsample_conditions_1
H(CACO)NCOsample_2isotropicsample_conditions_1

Software:

TOPSPIN v3 - collection, processing

NMRFAM-SPARKY v1.3 - peak picking

MARS v1.1 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE II+ 700 MHz
  • Bruker AVANCE III-HD 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks