BMRB Entry 52482

Name: Axin1 308-426
Published: 2025-09-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52482

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Axin1 308-426

Deposition date:

2024-05-23

Original release date:

2025-09-16

Authors:

Rutherford, Trevor; Li, Tie-Mei; Franco-Echevarria, Elsa; Gammons, Melissa; Bienz, Mariann

Citation:

Citation: Gammons, Melissa; Franco-Echevarria, Elsa; Li, Tie-Mei; Rutherford, Trevor; Renko, Miha; Batters, Christopher; Bienz, Mariann. "Wnt signalosome assembly is governed by conformational flexibility of Axin and by the AP2 clathrin adaptor" Nat. Commun. 16, 4718-4718 (2025).
PubMed: 40399324

Assembly members:

Assembly members:
entity_1, polymer, 119 residues, 13636 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AGYALAPATSANDSEQQSLS SDADTLSLTDSSVDGIPPYR IRKQHRREMQESVQVNGRVP LPHIPRTYRVPKEVRVEPQK FAEELIHRLEAVQRTREAEE KLEERLKRVRMEEEGEDGD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	336
15N chemical shifts	112
1H chemical shifts	218

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Axin1-A3	1

Entities:

Entity 1, Axin1-A3 119 residues - 13636 Da.

1

ALA

GLY

TYR

ALA

LEU

ALA

PRO

ALA

THR

SER

2

ALA

ASN

ASP

SER

GLU

GLN

SER

LEU

SER

3

SER

ASP

ALA

ASP

THR

LEU

SER

LEU

THR

ASP

4

SER

VAL

ASP

GLY

ILE

PRO

TYR

ARG

5

ILE

ARG

LYS

GLN

HIS

ARG

GLU

MET

GLN

6

GLU

SER

VAL

GLN

VAL

ASN

GLY

ARG

VAL

PRO

7

LEU

PRO

HIS

ILE

PRO

ARG

THR

TYR

ARG

VAL

8

PRO

LYS

GLU

VAL

ARG

VAL

GLU

PRO

GLN

LYS

9

PHE

ALA

GLU

LEU

ILE

HIS

ARG

LEU

GLU

10

ALA

VAL

GLN

ARG

THR

ARG

GLU

ALA

GLU

11

LYS

LEU

GLU

ARG

LEU

LYS

ARG

VAL

ARG

12

MET

GLU

GLY

GLU

ASP

GLY

ASP

Samples:

sample_1: Axin1-A3, [U-99% 13C; U-99% 15N], 500 uM; H2O 95%; D2O, [U-99% 2H], 5%; sodium phosphate 25 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 210 mM; pH: 6.7; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
HACAN	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3 - collection, processing

NMRFAM-SPARKY v1.3 - peak picking

MARS v1.1 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE II+ 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks