BMRB Entry 52478

Name: NMR chemical shift assignments of modified tRNAPhe from Escherichia coli
Published: 2024-10-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52478

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR chemical shift assignments of modified tRNAPhe from Escherichia coli

Deposition date:

2024-05-22

Original release date:

2024-10-22

Authors:

Yared, Marcel-Joseph; Chagneau, Carine; Barraud, Pierre

Citation:

Citation: Yared, Marcel-Joseph; Chagneau, Carine; Barraud, Pierre. "Imino chemical shift assignments of tRNAAsp, tRNAVal and tRNAPhe from Escherichia coli" Biomol. NMR Assignments 18, 323-331 (2024).
PubMed: 39365419

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GCCCGGAXAGCUCAGXCGGX AGAGCAGGGGAXUGAAXAXC CCCGUXXCCUUGGXXCGAUU CCGAGUCCGGGCACCA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	27
1H chemical shifts	33

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	modified tRNAPhe	1

Entities:

Entity 1, modified tRNAPhe 76 residues - Formula weight is not available

1

G

C

G

A

S4U

A

G

2

C

U

C

A

G

D

C

G

D

3

A

G

A

G

C

A

G

4

A

PSU

U

G

A

MS2I6A

A

PSU

C

5

C

G

U

M7G

ACP3U

C

U

6

U

G

M5U

PSU

C

G

A

U

7

C

G

A

G

U

C

G

8

G

C

A

C

A

Samples:

sample_1: modified tRNAPhe 0.53 mM; sodium phosphate 10 mM; magnesium chloride 10 mM; D2O 5%

sample_2: modified tRNAPhe, [U-98% 15N], 0.02 mM; sodium phosphate 10 mM; magnesium chloride 10 mM; D2O 5%

sample_conditions_1: ionic strength: 30 mM; pH: 6.0; pressure: 1 atm; temperature: 311 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1

Software:

TOPSPIN - collection, processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 950 MHz
Bruker AVANCE III 700 MHz