BMRB Entry 52468

Name: SY-tagged Tm1 I/C C-terminal Domain 13C 15N Fibril Assignments
Published: 2024-08-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52468

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SY-tagged Tm1 I/C C-terminal Domain 13C 15N Fibril Assignments

Deposition date:

2024-05-17

Original release date:

2024-08-15

Authors:

Fonda, Blake; Kato, Masato; Li, Yang; Murray, Dylan

Citation:

Citation: Fonda, Blake; Kato, Masato; Li, Yang; Murray, Dylan. "Cryo-EM and Solid State NMR Together Provide a More Comprehensive Structural Investigation of Protein Fibrils" bioRxiv ., .-. (2024).
PubMed: 38853912

Assembly members:

Assembly members:
entity_1, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHis-parellel1-GFP

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SYSSIKLSNNNNNSNSNNIE ISKSESCNASDIGGTNNNNA SRTIASAAVGEETSTLSSTS HEHNNNPNNDT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	168
15N chemical shifts	28

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tm1 I/C C-terminal Domain	1

Entities:

Entity 1, Tm1 I/C C-terminal Domain 71 residues - Formula weight is not available

1

SER

TYR

SER

ILE

LYS

LEU

SER

ASN

2

ASN

SER

ASN

SER

ASN

ILE

GLU

3

ILE

SER

LYS

SER

GLU

SER

CYS

ASN

ALA

SER

4

ASP

ILE

GLY

THR

ASN

ALA

5

SER

ARG

THR

ILE

ALA

SER

ALA

VAL

GLY

6

GLU

THR

SER

THR

LEU

SER

THR

SER

7

HIS

GLU

HIS

ASN

PRO

ASN

ASP

8

THR

Samples:

sample_1: Tropomyosin I/C Tail-Domain, [U-13C; U-15N], mM; sodium chloride 200 mM; PMSF 0.1 mM; EDTA 0.5 mM; TRIS 20 mM; sodium azide 0.02%; beta-mercaptoethanol 20 mM

sample_conditions_1: pH: 7.5; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-13C DARR	sample_1	isotropic	sample_conditions_1
2D NCACX	sample_1	isotropic	sample_conditions_1
2D NCOCX	sample_1	isotropic	sample_conditions_1
2D 13C/13C INEPT-TOBSY	sample_1	isotropic	sample_conditions_1
3D NCOCX	sample_1	isotropic	sample_conditions_1
3D NCACX	sample_1	isotropic	sample_conditions_1
3D CANCO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v10.6 - processing

NMRFAM-SPARKY v1.470 - peak picking

MCASSIGN v2b - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz