BMRB Entry 52455

Name: Backbone 1H, 15N, and 13C resonance assignments of FF1 domain from P190A RhoGAP in 5 M urea.
Published: 2024-10-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52455

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 15N, and 13C resonance assignments of FF1 domain from P190A RhoGAP in 5 M urea.

Deposition date:

2024-05-14

Original release date:

2024-10-22

Authors:

Camilo Ramos, Aarao; Pinheiro-Aguiar, Ramon; Almeida, Fabio

Citation:

Citation: Camilo-Ramos, Aarao; Korzhnev, Dmitry; Pinheiro-Aguiar, Ramon; Almeida, Fabio. "Backbone 1H, 15N, and 13C resonance assignments of the FF1 domain from P190A RhoGAP in 5 and 8 M urea" Biomol. NMR Assign. 18, 257-262 (2024).
PubMed: 39402262

Assembly members:

Assembly members:
entity_1, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET23

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MENLYFQGSQQIATAKDKYE WLVSRIVKNHNENWLSVSRK MQASPEYQDYVYLEGTQKAK KLFLQHIHRLKHE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	233
15N chemical shifts	77
1H chemical shifts	76

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FF1 domain	1

Entities:

Entity 1, FF1 domain 93 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

GLU

ASN

LEU

TYR

PHE

GLN

GLY

SER

GLN

4

GLN

ILE

ALA

THR

ALA

LYS

ASP

LYS

TYR

GLU

5

TRP

LEU

VAL

SER

ARG

ILE

VAL

LYS

ASN

HIS

6

ASN

GLU

ASN

TRP

LEU

SER

VAL

SER

ARG

LYS

7

MET

GLN

ALA

SER

PRO

GLU

TYR

GLN

ASP

TYR

8

VAL

TYR

LEU

GLU

GLY

THR

GLN

LYS

ALA

LYS

9

LYS

LEU

PHE

LEU

GLN

HIS

ILE

HIS

ARG

LEU

10

LYS

HIS

GLU

Samples:

sample_1: FF1 domain, [U-100% 13C; U-100% 15N], 600 ± 20 uM; Phosphate Buffer 50 mM; NaCl 150 mM; urea 5 M

sample_conditions_1: ionic strength: 268.6 mM; pH: 7.2; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCAN	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNN	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600.033 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks