BMRB Entry 52452

Name: Assignment of the Lassa virus glycoprotein 2 transmembrane domain G429P mutant in the postfusion state
Published: 2024-07-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52452

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of the Lassa virus glycoprotein 2 transmembrane domain G429P mutant in the postfusion state

Deposition date:

2024-05-10

Original release date:

2024-07-19

Authors:

Keating, Patrick; Lee, Jinwoo

Citation:

Citation: Keating, Patrick; Lee, Jinwoo. "Assignment of the Lassa virus transmembrane domain in the prefusion and postfusion states in detergent micelles" Biomol. NMR Assign. ., .-. (2024).
PubMed: 38916786

Assembly members:

Assembly members:
entity_1, polymer, 40 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mammarenavirus lassaense Taxonomy ID: 3052310 Superkingdom: Viruses Kingdom: not available Genus/species: Mammarenavirus lassaense

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSGKTPLPLVDLFVFSTSFY LISIFLHLVKIPTHRHIVGK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	76
15N chemical shifts	36
1H chemical shifts	36

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	G429P	1

Entities:

Entity 1, G429P 40 residues - Formula weight is not available

1	GLY	SER	GLY	LYS	THR	PRO	LEU	PRO	LEU	VAL
2	ASP	LEU	PHE	VAL	PHE	SER	THR	SER	PHE	TYR
3	LEU	ILE	SER	ILE	PHE	LEU	HIS	LEU	VAL	LYS
4	ILE	PRO	THR	HIS	ARG	HIS	ILE	VAL	GLY	LYS

Samples:

sample_1: Lassa virus transmembrane domain, [U-13C; U-15N], 400 uM; sodium chloride 100 mM; sodium phosphate 25 mM; LMPG 100 mM

sample_conditions_1: pH: 4.0; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRbox - data analysis

TOPSPIN - collection

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz

1	GLY	SER	GLY	LYS	THR	PRO	LEU	PRO	LEU	VAL
2	ASP	LEU	PHE	VAL	PHE	SER	THR	SER	PHE	TYR
3	LEU	ILE	SER	ILE	PHE	LEU	HIS	LEU	VAL	LYS
4	ILE	PRO	THR	HIS	ARG	HIS	ILE	VAL	GLY	LYS

1	GLY	SER	GLY	LYS	THR	PRO	LEU	PRO	LEU	VAL
2	ASP	LEU	PHE	VAL	PHE	SER	THR	SER	PHE	TYR
3	LEU	ILE	SER	ILE	PHE	LEU	HIS	LEU	VAL	LYS
4	ILE	PRO	THR	HIS	ARG	HIS	ILE	VAL	GLY	LYS