BMRB Entry 52446

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52446

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Title:
SARS-CoV-2 Fusion Domain: K825A Mutant
Deposition date:
2024-05-07
Original release date:
2024-07-19
Authors:
Birtles, Daniel; Lee, Jinwoo
Citation:

Citation: Birtles, Daniel; Guiyab, Lijon; Abbas, Wafa; Lee, Jinwoo. "Positive residues of the SARS-CoV-2 fusion domain are key contributors to the initiation of membrane fusion"  J. Biol. Chem. 300, 107564-107564 (2024).
PubMed: 39002677

Assembly members:

Assembly members:
entity_1, polymer, 40 residues, 4367.01 Da.

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SFIEDLLFNAVTLADAGFIK QYGDCLGDIAARDLICAQKF

Data sets:
Data typeCount
13C chemical shifts65
15N chemical shifts39
19F chemical shifts4
1H chemical shifts39

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SARS-CoV-2 Fusion Domain mutant K825A1

Entities:

Entity 1, SARS-CoV-2 Fusion Domain mutant K825A 40 residues - 4367.01 Da.

1   SERPFFILEGLUASPLEULEUPFFASNALA
2   VALTHRLEUALAASPALAGLYPFFILELYS
3   GLNTYRGLYASPCYSLEUGLYASPILEALA
4   ALAARGASPLEUILECYSALAGLNLYSPFF

Samples:

sample_1: SARS-CoV-2 Fusion Domain mutant K825A, [U-15N], 360 uM; SARS-CoV-2 Fusion Domain mutant K825A, [U-13C], 360 uM

sample_2: SARS-CoV-2 Fusion Domain mutant K825A, 19F-Phe, 360 uM

sample_conditions_1: pH: 5.0; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
1H 19Fsample_2isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

MestReNova - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks