BMRB Entry 52445
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52445
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Citation: Birtles, Daniel; Guiyab, Lijon; Abbas, Wafa; Lee, Jinwoo. "Positive residues of the SARS-CoV-2 fusion domain are key contributors to the initiation of membrane fusion" J. Biol. Chem. 300, 107564-107564 (2024).
PubMed: 39002677
Assembly members:
entity_1, polymer, 40 residues, 4424.11 Da.
Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41
Entity Sequences (FASTA):
entity_1: SFIEDLLFNKVTLADAGFIK
QYGDCLGDIAARDLICAQKF
| Data type | Count |
| 19F chemical shifts | 16 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SARS-CoV-2 Fusion Domain | 1 |
Entities:
Entity 1, SARS-CoV-2 Fusion Domain 40 residues - 4424.11 Da.
| 1 | SER | PFF | ILE | GLU | ASP | LEU | LEU | PFF | ASN | LYS | |
| 2 | VAL | THR | LEU | ALA | ASP | ALA | GLY | PFF | ILE | LYS | |
| 3 | GLN | TYR | GLY | ASP | CYS | LEU | GLY | ASP | ILE | ALA | |
| 4 | ALA | ARG | ASP | LEU | ILE | CYS | ALA | GLN | LYS | PFF |
Samples:
sample_1: SARS-CoV-2 Fusion Domain mutant K825A, 19F-Phe, 300 uM
sample_conditions_1: pH: 7.4; temperature: 296 K
sample_conditions_2: pH: 7.4; temperature: 296 K
sample_conditions_3: pH: 5.0; temperature: 296 K
sample_conditions_4: pH: 4.8; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H 19F | sample_1 | isotropic | sample_conditions_1 |
| 1H 19F | sample_1 | isotropic | sample_conditions_2 |
| 1H 19F | sample_1 | isotropic | sample_conditions_3 |
| 1H 19F | sample_1 | isotropic | sample_conditions_4 |
Software:
MestReNova - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz