BMRB Entry 52443

Name: Convergent evolution of intrinsically disordered regions in vertebrate galectins for liquid-liquid phase separation
Published: 2026-01-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52443

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Convergent evolution of intrinsically disordered regions in vertebrate galectins for liquid-liquid phase separation

Deposition date:

2024-05-05

Original release date:

2026-01-05

Authors:

Chen, Yu-Chen; Lin, Yu-Hao; Huang, Jie-rong

Citation:

Citation: Chen, Yu-Chen; Lin, Yu-Hao; Huang, Jie-rong. "Convergent evolution of intrinsically disordered regions in vertebrate galectins for liquid-liquid phase separation" .

Assembly members:

Assembly members:
entity_1, polymer, 141 residues, Formula weight is not available

Natural source:

Natural source: Common Name: zebrafish Taxonomy ID: 7955 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Danio rerio

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHD

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: QVLTVPLDFPLQNGAYNKML ITINGEVKPNAKQFTVNLRR GNDIAFHINPRFSEGGKPVI VRNSMIGNNWGREERELPSF PFVPGKPFEMKILITDTEYK VAVNKSHLLEFKHRVFELNQ ITGLSIYNDVTLSTVNVETL Q

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	360
15N chemical shifts	127
1H chemical shifts	127

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	zebrafish galectin	1

Entities:

Entity 1, zebrafish galectin 141 residues - Formula weight is not available

1

GLN

VAL

LEU

THR

VAL

PRO

LEU

ASP

PHE

PRO

2

LEU

GLN

ASN

GLY

ALA

TYR

ASN

LYS

MET

LEU

3

ILE

THR

ILE

ASN

GLY

GLU

VAL

LYS

PRO

ASN

4

ALA

LYS

GLN

PHE

THR

VAL

ASN

LEU

ARG

5

GLY

ASN

ASP

ILE

ALA

PHE

HIS

ILE

ASN

PRO

6

ARG

PHE

SER

GLU

GLY

LYS

PRO

VAL

ILE

7

VAL

ARG

ASN

SER

MET

ILE

GLY

ASN

TRP

8

GLY

ARG

GLU

ARG

GLU

LEU

PRO

SER

PHE

9

PRO

PHE

VAL

PRO

GLY

LYS

PRO

PHE

GLU

MET

10

LYS

ILE

LEU

ILE

THR

ASP

THR

GLU

TYR

LYS

11

VAL

ALA

VAL

ASN

LYS

SER

HIS

LEU

GLU

12

PHE

LYS

HIS

ARG

VAL

PHE

GLU

LEU

ASN

GLN

13

ILE

THR

GLY

LEU

SER

ILE

TYR

ASN

ASP

VAL

14

THR

LEU

SER

THR

VAL

ASN

VAL

GLU

THR

LEU

15

GLN

Samples:

sample_1: zebrafish galectin, [U-100% 13C; U-100% 15N], 100 uM

sample_conditions_1: ionic strength: 0 M; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift calculation, data analysis

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks