BMRB Entry 52440
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52440
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NMR-STAR v3 text file.
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Citation: Caldas Nogueira, Maria Luiza; Lakshmanan, Renuk; Riviere, Gwladys; Mietzsch, Mar; Bennett, Antonette; Mckenna, Robert; Long, Joanna. "Backbone NMR resonance assignments for the VP1u N-terminal receptor-binding domain of the human parvovirus pathogen B19" Biomol. NMR Assignments 18, 147-152 (2024).
PubMed: 38904726
Assembly members:
entity_1, polymer, 98 residues, Formula weight is not available
Natural source: Common Name: Human parvovirus B19 Taxonomy ID: 10798 Superkingdom: Viruses Kingdom: not available Genus/species: Erythroparvovirus Erythroparvovirus primate1
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30
Entity Sequences (FASTA):
entity_1: MSKESGKWWESDDEFAKAVY
QQFVEFYEKVTGTDLELIQI
LKDHYNISLDNPLENPSSLF
DLVARIKNNLKNSPDLYSHH
FQSHGQLSDHLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 256 |
| 15N chemical shifts | 78 |
| 1H chemical shifts | 327 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VP1u receptor biding domain | 1 |
Entities:
Entity 1, VP1u receptor biding domain 98 residues - Formula weight is not available
| 1 | MET | SER | LYS | GLU | SER | GLY | LYS | TRP | TRP | GLU | ||||
| 2 | SER | ASP | ASP | GLU | PHE | ALA | LYS | ALA | VAL | TYR | ||||
| 3 | GLN | GLN | PHE | VAL | GLU | PHE | TYR | GLU | LYS | VAL | ||||
| 4 | THR | GLY | THR | ASP | LEU | GLU | LEU | ILE | GLN | ILE | ||||
| 5 | LEU | LYS | ASP | HIS | TYR | ASN | ILE | SER | LEU | ASP | ||||
| 6 | ASN | PRO | LEU | GLU | ASN | PRO | SER | SER | LEU | PHE | ||||
| 7 | ASP | LEU | VAL | ALA | ARG | ILE | LYS | ASN | ASN | LEU | ||||
| 8 | LYS | ASN | SER | PRO | ASP | LEU | TYR | SER | HIS | HIS | ||||
| 9 | PHE | GLN | SER | HIS | GLY | GLN | LEU | SER | ASP | HIS | ||||
| 10 | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: VP1u receptor binding domain, [U-100% 13C; U-100% 15N], 450 uM; D2O 10%; sodium phosphate 20 mM; sodium chloride 50 mM; arginine 25 mM; Glutamic acid 25 mM; Trimethylsilylpropanoic acid 112 uM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCOCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v3.2 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks