BMRB Entry 5244

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5244

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Assignment of Endonuclease V from Bacteriophage T4 with deuterium labeling

Deposition date:

2001-12-26

Original release date:

2002-08-22

Authors:

Ahn, Hee-Chul; Lee, Sung-Yun; Lee, Bong-Jin

Citation:

Citation: Ahn, Hee-Chul; Lee, Sung-Yun; Lee, Bong-Jin. "Letter to the Editor: Backbone Assignments for Endonuclease V from Bacteriophage T4 with deuterium labeling " J. Biomol. NMR 22, 383-384 (2002).
PubMed: 12018491

Assembly members:

Assembly members:
endonuclease V, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source: Common Name: T4 Taxonomy ID: 10665 Superkingdom: Viruses Kingdom: not available Genus/species: T4-like phages Bacteriophage T4

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
endonuclease V: MTRINLTLVSELADQHLMAE YRELPRVFGAVRKHVANGKR VRDFKISPTFILGAGHVTFF YDKLEFLRKRQIELIAECLK RGFNIKDTTVQDISDIPQEF RGDYIPHEASIAISQARLDE KIAQRPTWYKYYGKAIYA

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	253
13C chemical shifts	395
15N chemical shifts	131

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	endonuclease V	1

Entities:

Entity 1, endonuclease V 138 residues - Formula weight is not available

1

MET

THR

ARG

ILE

ASN

LEU

THR

LEU

VAL

SER

2

GLU

LEU

ALA

ASP

GLN

HIS

LEU

MET

ALA

GLU

3

TYR

ARG

GLU

LEU

PRO

ARG

VAL

PHE

GLY

ALA

4

VAL

ARG

LYS

HIS

VAL

ALA

ASN

GLY

LYS

ARG

5

VAL

ARG

ASP

PHE

LYS

ILE

SER

PRO

THR

PHE

6

ILE

LEU

GLY

ALA

GLY

HIS

VAL

THR

PHE

7

TYR

ASP

LYS

LEU

GLU

PHE

LEU

ARG

LYS

ARG

8

GLN

ILE

GLU

LEU

ILE

ALA

GLU

CYS

LEU

LYS

9

ARG

GLY

PHE

ASN

ILE

LYS

ASP

THR

VAL

10

GLN

ASP

ILE

SER

ASP

ILE

PRO

GLN

GLU

PHE

11

ARG

GLY

ASP

TYR

ILE

PRO

HIS

GLU

ALA

SER

12

ILE

ALA

ILE

SER

GLN

ALA

ARG

LEU

ASP

GLU

13

LYS

ILE

ALA

GLN

ARG

PRO

THR

TRP

TYR

LYS

14

TYR

GLY

LYS

ALA

ILE

TYR

ALA

Samples:

sample_1: endonuclease V, [U-15N]-Ala, Asp, Arg, Gly, Lys, Ser, Thr, Tyr, Val, 1.0 mM

sample_2: endonuclease V, [U-15N], 2.0 mM

sample_3: endonuclease V, [U-70% 2H ; U-15N], 1.5 mM

sample_4: endonuclease V, [U-90% 2H; U-13C; U-15N], 1.5 mM

Ex-cond: pH: 6.5; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	not available	not available
3D 1H-15N NOESY	not available	not available	not available
3D 1H-15N TOCSY	not available	not available	not available
3D HNHA	not available	not available	not available
3D HNCA	not available	not available	not available
3D HN(CO)CA	not available	not available	not available
3D HNCACB	not available	not available	not available
3D HN(CO)CACB	not available	not available	not available
3D HNCO	not available	not available	not available

Software:

NMRPipe - data processing

NMRDraw - data processing

NMRView - peak assignments

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz

PDB	1ENI 1ENJ 1ENK 1VAS 2END 2FCC
EMBL	CAA28215
GB	AAA72721 AAC42096 AAD42563 ABX11097 ACL78337
REF	NP_049733 YP_001595242 YP_002922460 YP_003734258
SP	P04418
AlphaFold	P04418