BMRB Entry 52439

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52439

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Title:
NMR assignment of the S254D/S260D variant of human Nucleophosmin DNA-binding domain
Deposition date:
2024-05-01
Original release date:
2026-01-26
Authors:
Rivero-Garcia, Pablo; Giner-Arroyo, Rafael; Tamargo-Azpilicueta, Joaquin; Telfer, Abbey; Frezza, Elisa; Velazquez-Campoy, Adrian; Diaz-Moreno, Sofia; De la Rosa, Miguel; Diaz-Moreno, Irene
Citation:

Citation: Rivero-Garcia, Pablo; Giner-Arroyo, Rafael; Tamargo-Azpilicueta, Joaquin; Telfer, Abbey; Frezza, Elisa; Velazquez-Campoy, Adrian; Diaz-Moreno, Sofia; De la Rosa, Miguel; Diaz-Moreno, Irene. "Multisite phosphorylation of the AML-linked C-terminal of nucleophosmin (NPM1) orchestrates protein stability, DNA binding, and charge block-driven phase separation"  Nucleic Acids Res. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 90 residues, 10309.73 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28+(a)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MESFKKQEKTPKTPKGPSSV EDIKAKMQADIEKGGDLPKV EAKFINYVKNCFRMTDQEAI QDLWQWRKSL

Data sets:
Data typeCount
15N chemical shifts58
1H chemical shifts58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NPM1 C70 S254D/S260D1

Entities:

Entity 1, NPM1 C70 S254D/S260D 90 residues - 10309.73 Da.

Polyhistidine tag is included in the reference sequence, although not included in the assignment.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLUSERPHELYSLYSGLNGLULYSTHR
4   PROLYSTHRPROLYSGLYPROSERSERVAL
5   GLUASPILELYSALALYSMETGLNALAASP
6   ILEGLULYSGLYGLYASPLEUPROLYSVAL
7   GLUALALYSPHEILEASNTYRVALLYSASN
8   CYSPHEARGMETTHRASPGLNGLUALAILE
9   GLNASPLEUTRPGLNTRPARGLYSSERLEU

Samples:

sample_1: NPM1 C70 S254D/S260D, [U-100% 15N], 500 uM; D2O, [U-100% 2H], 5%; sodium phosphate 20 mM; TCEP 2.5 mM

sample_2: NPM1 C70 S254D/S260D, [U-100% 15N], 650 uM; D2O, [U-100% 2H], 5%; sodium phosphate 20 mM; TCEP 3 mM

sample_conditions_1: ionic strength: 20 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESY-HSQCsample_2isotropicsample_conditions_1
3D 1H-15N TOCSY-HSQCsample_2isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment, chemical shift calculation, peak picking

TOPSPIN v4.2 - collection

Origin v8.6 - chemical shift calculation, data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks