BMRB Entry 52425

Name: Helix-I of the Programmed Ribosome Frameshift (PRF) element of SARS-CoV-2
Published: 2024-07-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52425

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Helix-I of the Programmed Ribosome Frameshift (PRF) element of SARS-CoV-2

Deposition date:

2024-04-29

Original release date:

2024-07-29

Authors:

Hernandez-Marin, Maria; Cantero-Camacho, Angel; Lopez-Nunez, Sergio; Gallego, Jose

Citation:

Citation: Hernandez-Marin, Maria; Cantero-Camacho, Angel; Mena, Ignacio; Lopez-Nunez, Sergio; Garcia-Sastre, Adolfo; Gallego, Jose. "Sarbecovirus programmed ribosome frameshift RNA element folding studied by NMR spectroscopy and comparative analyses" Nucleic Acids Res 52, 11960-11972 (2024).
PubMed: 39149904

Assembly members:

Assembly members:
entity_1, polymer, 56 residues, Formula weight is not available

Natural source:

Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source: Production method: in vitro transcription Host organism: Not applicable

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGGCGAACCCAUGCUUCAGU CAGCUGAUGCACAAUCGUUU UUAAACGGGUUUGCCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	36
1H chemical shifts	41

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Helix-I of SARS-CoV-2 PRF	1

Entities:

Entity 1, Helix-I of SARS-CoV-2 PRF 56 residues - Formula weight is not available

The sequences experimentally analyzed in this study correspond to SARS-CoV-2 isolate Wuhan-Hu-1 (NCBI NC_045512). To obtain genomic numbering add 13,419 to the authors' sequence numbers.

1

G

C

G

A

C

2

A

U

G

C

U

C

A

G

U

3

C

A

G

C

U

G

A

U

G

C

4

A

C

A

U

C

G

U

5

U

A

C

G

U

6

U

G

C

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC NH2 only	sample_1	isotropic	sample_conditions_1
2D 1H-15N HNN-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

BMRB Entry 52425

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: