BMRB Entry 52423
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52423
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Zhou, Bing-Rui; Feng, Hanqiao; Huang, Furong; Zhu, Iris; Portillo-Ledesma, Stephanie; Shi, Dan; Zaret, Kenneth; Schlick, Tamar; Landsman, David; Wang, Qianben; Bai, Yawen. "Structural insights into the cooperative nucleosome recognition and chromatin opening by FOXA1 and GATA4" Mol. Cell 84, 3061-3079 (2024).
PubMed: 39121853
Assembly members:
FoxA1, polymer, 331 residues, Formula weight is not available
H2A, polymer, 130 residues, Formula weight is not available
H2B, polymer, 126 residues, Formula weight is not available
H3, polymer, 136 residues, Formula weight is not available
H4, polymer, 103 residues, Formula weight is not available
186bp N1DNA, polymer, 186 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29b
Entity Sequences (FASTA):
FoxA1: AYAPSNLGRSRAGGGGDAKT
FKRSYPHAKPPYSYISLITM
AIQQAPSKMLTLSEIYQWIM
DLFPYYRQNQQRWQNSIRHS
LSFNDCFVKVARSPDKPGKG
SYWTLHPDSGNMFENGCYLR
RQKRFKCEKQPGAGGGGGSG
SGGSGAKGGPESRKDPSGAS
NPSADSPLHRGVHGKTGQLE
GAPAPGPAASPQTLDHSGAT
ATGGASELKTPASSTAPPIS
SGPGALASVPASHPAHGLAP
HESQLHLKGDPHYSFNHPFS
INNLMSSSEQQHKLDFKAYE
QALQYSPYGSTLPASLPLGS
ASVTTRSPIEPSALEPAYYQ
GVYSRPVLNTS
H2A: MSGRGKQGGKARAKAKTRSS
RAGLQFPVGRVHRLLRKGNY
SERVGAGAPVYLAAVLEYLT
AEILELAGNAARDNKKTRII
PRHLQLAIRNDEELNKLLGR
VTIAQGGVLPNIQAVLLPKK
TESHHKAKGK
H2B: MPEPAKSAPAPKKGSKKAVT
KAQKKDGKKRKRSRKESYSI
YVYKVLKQVHPDTGISSKAM
GIMNSFVNDIFERIAGEASR
LAHYNKRSTITSREIQTAVR
LLLPGELAKHAVSEGTKAVT
KYTSAK
H3: MARTKQTARKSTGGKAPRKQ
LATKAARKSAPATGGVKKPH
RYRPGTVALREIRRYQKSTE
LLIRKLPFQRLVREIAQDFK
TDLRFQSSAVMALQEACEAY
LVGLFEDTNLCAIHAKRVTI
MPKDIQLARRIRGERA
H4: MSGRGKGGKGLGKGGAKRHR
KVLRDNIQGITKPAIRRLAR
RGGVKRISGLIYEETRGVLK
VFLENVIRDAVTYTEHAKRK
TVTAMDVVYALKRQGRTLYG
FGG
186bp N1DNA: ATCCGAGATGGTACTTTGTG
TCTCCTGCTCTGTCAGCAGG
GCACTGTACTTGCTGATACC
AGGGAATGTTTGTTCTTAAA
TACCATCATTCCGGACGTGT
TTGCCTTGGCCAGTTTTCCA
TGTACATGCAGAAAGAAGTT
TGGACTGATCAATACAGTCC
TCTGCCTTTAAAGCAATAGG
AAAGAT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 245 |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 77 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FoxA1 | 1 |
| 2 | H2A | 2 |
| 3 | H2B | 3 |
| 4 | H3 | 4 |
| 5 | H4 | 5 |
| 6 | 186bp N1DNA | 6 |
Entities:
Entity 1, FoxA1 331 residues - Formula weight is not available
| 1 | ALA | TYR | ALA | PRO | SER | ASN | LEU | GLY | ARG | SER | ||||
| 2 | ARG | ALA | GLY | GLY | GLY | GLY | ASP | ALA | LYS | THR | ||||
| 3 | PHE | LYS | ARG | SER | TYR | PRO | HIS | ALA | LYS | PRO | ||||
| 4 | PRO | TYR | SER | TYR | ILE | SER | LEU | ILE | THR | MET | ||||
| 5 | ALA | ILE | GLN | GLN | ALA | PRO | SER | LYS | MET | LEU | ||||
| 6 | THR | LEU | SER | GLU | ILE | TYR | GLN | TRP | ILE | MET | ||||
| 7 | ASP | LEU | PHE | PRO | TYR | TYR | ARG | GLN | ASN | GLN | ||||
| 8 | GLN | ARG | TRP | GLN | ASN | SER | ILE | ARG | HIS | SER | ||||
| 9 | LEU | SER | PHE | ASN | ASP | CYS | PHE | VAL | LYS | VAL | ||||
| 10 | ALA | ARG | SER | PRO | ASP | LYS | PRO | GLY | LYS | GLY | ||||
| 11 | SER | TYR | TRP | THR | LEU | HIS | PRO | ASP | SER | GLY | ||||
| 12 | ASN | MET | PHE | GLU | ASN | GLY | CYS | TYR | LEU | ARG | ||||
| 13 | ARG | GLN | LYS | ARG | PHE | LYS | CYS | GLU | LYS | GLN | ||||
| 14 | PRO | GLY | ALA | GLY | GLY | GLY | GLY | GLY | SER | GLY | ||||
| 15 | SER | GLY | GLY | SER | GLY | ALA | LYS | GLY | GLY | PRO | ||||
| 16 | GLU | SER | ARG | LYS | ASP | PRO | SER | GLY | ALA | SER | ||||
| 17 | ASN | PRO | SER | ALA | ASP | SER | PRO | LEU | HIS | ARG | ||||
| 18 | GLY | VAL | HIS | GLY | LYS | THR | GLY | GLN | LEU | GLU | ||||
| 19 | GLY | ALA | PRO | ALA | PRO | GLY | PRO | ALA | ALA | SER | ||||
| 20 | PRO | GLN | THR | LEU | ASP | HIS | SER | GLY | ALA | THR | ||||
| 21 | ALA | THR | GLY | GLY | ALA | SER | GLU | LEU | LYS | THR | ||||
| 22 | PRO | ALA | SER | SER | THR | ALA | PRO | PRO | ILE | SER | ||||
| 23 | SER | GLY | PRO | GLY | ALA | LEU | ALA | SER | VAL | PRO | ||||
| 24 | ALA | SER | HIS | PRO | ALA | HIS | GLY | LEU | ALA | PRO | ||||
| 25 | HIS | GLU | SER | GLN | LEU | HIS | LEU | LYS | GLY | ASP | ||||
| 26 | PRO | HIS | TYR | SER | PHE | ASN | HIS | PRO | PHE | SER | ||||
| 27 | ILE | ASN | ASN | LEU | MET | SER | SER | SER | GLU | GLN | ||||
| 28 | GLN | HIS | LYS | LEU | ASP | PHE | LYS | ALA | TYR | GLU | ||||
| 29 | GLN | ALA | LEU | GLN | TYR | SER | PRO | TYR | GLY | SER | ||||
| 30 | THR | LEU | PRO | ALA | SER | LEU | PRO | LEU | GLY | SER | ||||
| 31 | ALA | SER | VAL | THR | THR | ARG | SER | PRO | ILE | GLU | ||||
| 32 | PRO | SER | ALA | LEU | GLU | PRO | ALA | TYR | TYR | GLN | ||||
| 33 | GLY | VAL | TYR | SER | ARG | PRO | VAL | LEU | ASN | THR | ||||
| 34 | SER |
Entity 2, H2A 130 residues - Formula weight is not available
| 1 | MET | SER | GLY | ARG | GLY | LYS | GLN | GLY | GLY | LYS | |
| 2 | ALA | ARG | ALA | LYS | ALA | LYS | THR | ARG | SER | SER | |
| 3 | ARG | ALA | GLY | LEU | GLN | PHE | PRO | VAL | GLY | ARG | |
| 4 | VAL | HIS | ARG | LEU | LEU | ARG | LYS | GLY | ASN | TYR | |
| 5 | SER | GLU | ARG | VAL | GLY | ALA | GLY | ALA | PRO | VAL | |
| 6 | TYR | LEU | ALA | ALA | VAL | LEU | GLU | TYR | LEU | THR | |
| 7 | ALA | GLU | ILE | LEU | GLU | LEU | ALA | GLY | ASN | ALA | |
| 8 | ALA | ARG | ASP | ASN | LYS | LYS | THR | ARG | ILE | ILE | |
| 9 | PRO | ARG | HIS | LEU | GLN | LEU | ALA | ILE | ARG | ASN | |
| 10 | ASP | GLU | GLU | LEU | ASN | LYS | LEU | LEU | GLY | ARG | |
| 11 | VAL | THR | ILE | ALA | GLN | GLY | GLY | VAL | LEU | PRO | |
| 12 | ASN | ILE | GLN | ALA | VAL | LEU | LEU | PRO | LYS | LYS | |
| 13 | THR | GLU | SER | HIS | HIS | LYS | ALA | LYS | GLY | LYS |
Entity 3, H2B 126 residues - Formula weight is not available
| 1 | MET | PRO | GLU | PRO | ALA | LYS | SER | ALA | PRO | ALA | ||||
| 2 | PRO | LYS | LYS | GLY | SER | LYS | LYS | ALA | VAL | THR | ||||
| 3 | LYS | ALA | GLN | LYS | LYS | ASP | GLY | LYS | LYS | ARG | ||||
| 4 | LYS | ARG | SER | ARG | LYS | GLU | SER | TYR | SER | ILE | ||||
| 5 | TYR | VAL | TYR | LYS | VAL | LEU | LYS | GLN | VAL | HIS | ||||
| 6 | PRO | ASP | THR | GLY | ILE | SER | SER | LYS | ALA | MET | ||||
| 7 | GLY | ILE | MET | ASN | SER | PHE | VAL | ASN | ASP | ILE | ||||
| 8 | PHE | GLU | ARG | ILE | ALA | GLY | GLU | ALA | SER | ARG | ||||
| 9 | LEU | ALA | HIS | TYR | ASN | LYS | ARG | SER | THR | ILE | ||||
| 10 | THR | SER | ARG | GLU | ILE | GLN | THR | ALA | VAL | ARG | ||||
| 11 | LEU | LEU | LEU | PRO | GLY | GLU | LEU | ALA | LYS | HIS | ||||
| 12 | ALA | VAL | SER | GLU | GLY | THR | LYS | ALA | VAL | THR | ||||
| 13 | LYS | TYR | THR | SER | ALA | LYS |
Entity 4, H3 136 residues - Formula weight is not available
| 1 | MET | ALA | ARG | THR | LYS | GLN | THR | ALA | ARG | LYS | ||||
| 2 | SER | THR | GLY | GLY | LYS | ALA | PRO | ARG | LYS | GLN | ||||
| 3 | LEU | ALA | THR | LYS | ALA | ALA | ARG | LYS | SER | ALA | ||||
| 4 | PRO | ALA | THR | GLY | GLY | VAL | LYS | LYS | PRO | HIS | ||||
| 5 | ARG | TYR | ARG | PRO | GLY | THR | VAL | ALA | LEU | ARG | ||||
| 6 | GLU | ILE | ARG | ARG | TYR | GLN | LYS | SER | THR | GLU | ||||
| 7 | LEU | LEU | ILE | ARG | LYS | LEU | PRO | PHE | GLN | ARG | ||||
| 8 | LEU | VAL | ARG | GLU | ILE | ALA | GLN | ASP | PHE | LYS | ||||
| 9 | THR | ASP | LEU | ARG | PHE | GLN | SER | SER | ALA | VAL | ||||
| 10 | MET | ALA | LEU | GLN | GLU | ALA | CYS | GLU | ALA | TYR | ||||
| 11 | LEU | VAL | GLY | LEU | PHE | GLU | ASP | THR | ASN | LEU | ||||
| 12 | CYS | ALA | ILE | HIS | ALA | LYS | ARG | VAL | THR | ILE | ||||
| 13 | MET | PRO | LYS | ASP | ILE | GLN | LEU | ALA | ARG | ARG | ||||
| 14 | ILE | ARG | GLY | GLU | ARG | ALA |
Entity 5, H4 103 residues - Formula weight is not available
| 1 | MET | SER | GLY | ARG | GLY | LYS | GLY | GLY | LYS | GLY | ||||
| 2 | LEU | GLY | LYS | GLY | GLY | ALA | LYS | ARG | HIS | ARG | ||||
| 3 | LYS | VAL | LEU | ARG | ASP | ASN | ILE | GLN | GLY | ILE | ||||
| 4 | THR | LYS | PRO | ALA | ILE | ARG | ARG | LEU | ALA | ARG | ||||
| 5 | ARG | GLY | GLY | VAL | LYS | ARG | ILE | SER | GLY | LEU | ||||
| 6 | ILE | TYR | GLU | GLU | THR | ARG | GLY | VAL | LEU | LYS | ||||
| 7 | VAL | PHE | LEU | GLU | ASN | VAL | ILE | ARG | ASP | ALA | ||||
| 8 | VAL | THR | TYR | THR | GLU | HIS | ALA | LYS | ARG | LYS | ||||
| 9 | THR | VAL | THR | ALA | MET | ASP | VAL | VAL | TYR | ALA | ||||
| 10 | LEU | LYS | ARG | GLN | GLY | ARG | THR | LEU | TYR | GLY | ||||
| 11 | PHE | GLY | GLY |
Entity 6, 186bp N1DNA 186 residues - Formula weight is not available
| 1 | DA | DT | DC | DC | DG | DA | DG | DA | DT | DG | ||||
| 2 | DG | DT | DA | DC | DT | DT | DT | DG | DT | DG | ||||
| 3 | DT | DC | DT | DC | DC | DT | DG | DC | DT | DC | ||||
| 4 | DT | DG | DT | DC | DA | DG | DC | DA | DG | DG | ||||
| 5 | DG | DC | DA | DC | DT | DG | DT | DA | DC | DT | ||||
| 6 | DT | DG | DC | DT | DG | DA | DT | DA | DC | DC | ||||
| 7 | DA | DG | DG | DG | DA | DA | DT | DG | DT | DT | ||||
| 8 | DT | DG | DT | DT | DC | DT | DT | DA | DA | DA | ||||
| 9 | DT | DA | DC | DC | DA | DT | DC | DA | DT | DT | ||||
| 10 | DC | DC | DG | DG | DA | DC | DG | DT | DG | DT | ||||
| 11 | DT | DT | DG | DC | DC | DT | DT | DG | DG | DC | ||||
| 12 | DC | DA | DG | DT | DT | DT | DT | DC | DC | DA | ||||
| 13 | DT | DG | DT | DA | DC | DA | DT | DG | DC | DA | ||||
| 14 | DG | DA | DA | DA | DG | DA | DA | DG | DT | DT | ||||
| 15 | DT | DG | DG | DA | DC | DT | DG | DA | DT | DC | ||||
| 16 | DA | DA | DT | DA | DC | DA | DG | DT | DC | DC | ||||
| 17 | DT | DC | DT | DG | DC | DC | DT | DT | DT | DA | ||||
| 18 | DA | DA | DG | DC | DA | DA | DT | DA | DG | DG | ||||
| 19 | DA | DA | DA | DG | DA | DT |
Samples:
sample_1: FoxA1(141-472 aa), [U-100% 13C; U-100% 15N], 350 uM; FoxA1(141-472 aa), [U-100% 15N], 350 uM; NaPi 20 mM; NaCl 50 mM
sample_conditions_1: ionic strength: 75 mM; pH: 6.3; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, NMRviewJ - data processing and chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks