BMRB Entry 52422

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52422

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Title:
Structural insights into the cooperative nucleosome recognition and chromatin opening by FOXA1 and GATA4
Deposition date:
2024-04-23
Original release date:
2024-08-22
Authors:
Zhou, Bing-Rui; Feng, Hanqiao; Huang, Furong; Zhu, Iris; Shi, Dan; Zaret, Kenneth; Landsman, David; Wang, Qianben; Bai, Yawen
Citation:

Citation: Zhou, Bing-Rui; Feng, Hanqiao; Huang, Furong; Zhu, Iris; Portillo-Ledesma, Stephanie; Shi, Dan; Zaret, Kenneth; Schlick, Tamar; Landsman, David; Wang, Qianben; Bai, Yawen. "Structural insights into the cooperative nucleosome recognition and chromatin opening by FOXA1 and GATA4"  Mol. Cell ., .-. (2024).
PubMed: 39121853

Assembly members:

Assembly members:
FoxA1, polymer, 331 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FoxA1: AYAPSNLGRSRAGGGGDAKT FKRSYPHAKPPYSYISLITM AIQQAPSKMLTLSEIYQWIM DLFPYYRQNQQRWQNSIRHS LSFNDCFVKVARSPDKPGKG SYWTLHPDSGNMFENGCYLR RQKRFKCEKQPGAGGGGGSG SGGSGAKGGPESRKDPSGAS NPSADSPLHRGVHGKTGQLE GAPAPGPAASPQTLDHSGAT ATGGASELKTPASSTAPPIS SGPGALASVPASHPAHGLAP HESQLHLKGDPHYSFNHPFS INNLMSSSEQQHKLDFKAYE QALQYSPYGSTLPASLPLGS ASVTTRSPIEPSALEPAYYQ GVYSRPVLNTS

Data sets:
Data typeCount
13C chemical shifts516
15N chemical shifts157
1H chemical shifts157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FoxA11

Entities:

Entity 1, FoxA1 331 residues - Formula weight is not available

1   ALATYRALAPROSERASNLEUGLYARGSER
2   ARGALAGLYGLYGLYGLYASPALALYSTHR
3   PHELYSARGSERTYRPROHISALALYSPRO
4   PROTYRSERTYRILESERLEUILETHRMET
5   ALAILEGLNGLNALAPROSERLYSMETLEU
6   THRLEUSERGLUILETYRGLNTRPILEMET
7   ASPLEUPHEPROTYRTYRARGGLNASNGLN
8   GLNARGTRPGLNASNSERILEARGHISSER
9   LEUSERPHEASNASPCYSPHEVALLYSVAL
10   ALAARGSERPROASPLYSPROGLYLYSGLY
11   SERTYRTRPTHRLEUHISPROASPSERGLY
12   ASNMETPHEGLUASNGLYCYSTYRLEUARG
13   ARGGLNLYSARGPHELYSCYSGLULYSGLN
14   PROGLYALAGLYGLYGLYGLYGLYSERGLY
15   SERGLYGLYSERGLYALALYSGLYGLYPRO
16   GLUSERARGLYSASPPROSERGLYALASER
17   ASNPROSERALAASPSERPROLEUHISARG
18   GLYVALHISGLYLYSTHRGLYGLNLEUGLU
19   GLYALAPROALAPROGLYPROALAALASER
20   PROGLNTHRLEUASPHISSERGLYALATHR
21   ALATHRGLYGLYALASERGLULEULYSTHR
22   PROALASERSERTHRALAPROPROILESER
23   SERGLYPROGLYALALEUALASERVALPRO
24   ALASERHISPROALAHISGLYLEUALAPRO
25   HISGLUSERGLNLEUHISLEULYSGLYASP
26   PROHISTYRSERPHEASNHISPROPHESER
27   ILEASNASNLEUMETSERSERSERGLUGLN
28   GLNHISLYSLEUASPPHELYSALATYRGLU
29   GLNALALEUGLNTYRSERPROTYRGLYSER
30   THRLEUPROALASERLEUPROLEUGLYSER
31   ALASERVALTHRTHRARGSERPROILEGLU
32   PROSERALALEUGLUPROALATYRTYRGLN
33   GLYVALTYRSERARGPROVALLEUASNTHR
34   SER

Samples:

sample_1: FoxA1(141-472 aa), [U-100% 13C; U-100% 15N], 350 uM; FoxA1(141-472 aa), [U-100% 15N], 350 uM; NaPi 20 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 75 mM; pH: 6.3; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3DHCANNHsample_1isotropicsample_conditions_1

Software:

NMRPipe, NMRviewJ - data processing and chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks