BMRB Entry 52421
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52421
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Citation: Karunatilleke, Nadun; Brickenden, Anne; Choy, Wing-Yiu. "Molecular basis of the interactions between the disordered Neh4 and Neh5 domains of Nrf2 and CBP/p300 in oxidative stress response" Protein Sci. 33, e5137-e5137 (2024).
PubMed: 39150085
Assembly members:
entity_1, polymer, 66 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST17
Entity Sequences (FASTA):
entity_1: VFIATNQAQSPETSVAQVAP
VDLDGMQQDIEQVWEELLSI
PELQCLNIENDKLVETTMVP
SPEAKL
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T1rho_relaxation
| Data type | Count |
| 13C chemical shifts | 119 |
| 15N chemical shifts | 48 |
| 1H chemical shifts | 48 |
| T1 relaxation values | 40 |
| T1rho relaxation values | 40 |
| heteronuclear NOE values | 40 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Neh5ex | 1 |
Entities:
Entity 1, Neh5ex 66 residues - Formula weight is not available
Residues 155-220 of human Nrf2
| 1 | VAL | PHE | ILE | ALA | THR | ASN | GLN | ALA | GLN | SER | ||||
| 2 | PRO | GLU | THR | SER | VAL | ALA | GLN | VAL | ALA | PRO | ||||
| 3 | VAL | ASP | LEU | ASP | GLY | MET | GLN | GLN | ASP | ILE | ||||
| 4 | GLU | GLN | VAL | TRP | GLU | GLU | LEU | LEU | SER | ILE | ||||
| 5 | PRO | GLU | LEU | GLN | CYS | LEU | ASN | ILE | GLU | ASN | ||||
| 6 | ASP | LYS | LEU | VAL | GLU | THR | THR | MET | VAL | PRO | ||||
| 7 | SER | PRO | GLU | ALA | LYS | LEU |
Samples:
sample_1: Neh5ex, [U-13C; U-15N], 400 uM; MES 20 mM; DTT 1 mM
sample_2: Neh5ex, [U-15N], 300 uM; MES 20 mM; DTT 1 mM
sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| T1/R1 relaxation | sample_2 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation | sample_2 | isotropic | sample_conditions_1 |
| 1H-15N heteronoe | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRDraw - data analysis
NMRViewJ - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated peaks