BMRB Entry 52420

Name: Neh4ex backbone chemical shifts
Published: 2024-08-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52420

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Neh4ex backbone chemical shifts

Deposition date:

2024-04-22

Original release date:

2024-08-20

Authors:

Karunatilleke, Nadun; Choy, Wing-Yiu

Citation:

Citation: Karunatilleke, Nadun; Brickenden, Anne; Choy, Wing-Yiu. "Molecular basis of the interactions between the disordered Neh4 and Neh5 domains of Nrf2 and CBP/p300 in oxidative stress response" Protein Sci. 33, e5137-e5137 (2024).
PubMed: 39150085

Assembly members:

Assembly members:
entity_1, polymer, 63 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST17

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSANYSQVAHIPKSDALYFD DCMQLLAQTFPFVDDNEVSS ATFQSLVPDIPGHIESPVFI ATN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
heteronucl_NOEs
- heteronucl_NOEs_1
heteronucl_T1_relaxation
- heteronucl_T1_relaxation_1
heteronucl_T1rho_relaxation
- heteronucl_T1rho_relaxation_1

Data type	Count
13C chemical shifts	121
15N chemical shifts	55
1H chemical shifts	55
T1 relaxation values	47
T1rho relaxation values	47
heteronuclear NOE values	46

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Neh4ex	1

Entities:

Entity 1, Neh4ex 63 residues - Formula weight is not available

Residues 99-160 of human Nrf2

1

GLY

SER

ALA

ASN

TYR

SER

GLN

VAL

ALA

HIS

2

ILE

PRO

LYS

SER

ASP

ALA

LEU

TYR

PHE

ASP

3

ASP

CYS

MET

GLN

LEU

ALA

GLN

THR

PHE

4

PRO

PHE

VAL

ASP

ASN

GLU

VAL

SER

5

ALA

THR

PHE

GLN

SER

LEU

VAL

PRO

ASP

ILE

6

PRO

GLY

HIS

ILE

GLU

SER

PRO

VAL

PHE

ILE

7

ALA

THR

ASN

Samples:

sample_1: Neh4ex, [U-13C; U-15N], 280 uM; MES 20 mM; DTT 1 mM

sample_2: Neh4ex, [U-15N], 300 uM; MES 20 mM; DTT 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
1H-15N heteronoe	sample_2	isotropic	sample_conditions_1
T1/R1 relaxation	sample_2	isotropic	sample_conditions_1
T1rho/R1rho relaxation	sample_2	isotropic	sample_conditions_1

Software:

NMRViewJ - chemical shift assignment

NMRDraw - Peak intensity measurements

NMR spectrometers:

Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks