BMRB Entry 52420

Title:
Neh4ex backbone chemical shifts
Deposition date:
2024-04-22
Original release date:
2024-08-20
Authors:
Karunatilleke, Nadun; Choy, Wing-Yiu
Citation:

Citation: Karunatilleke, Nadun; Brickenden, Anne; Choy, Wing-Yiu. "Molecular basis of the interactions between the disordered Neh4 and Neh5 domains of Nrf2 and CBP/p300 in oxidative stress response"  Protein Sci. 33, e5137-e5137 (2024).
PubMed: 39150085

Assembly members:

Assembly members:
entity_1, polymer, 63 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST17

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts121
15N chemical shifts55
1H chemical shifts55
T1 relaxation values47
T1rho relaxation values47
heteronuclear NOE values46

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Neh4ex1

Entities:

Entity 1, Neh4ex 63 residues - Formula weight is not available

Residues 99-160 of human Nrf2

1   GLYSERALAASNTYRSERGLNVALALAHIS
2   ILEPROLYSSERASPALALEUTYRPHEASP
3   ASPCYSMETGLNLEULEUALAGLNTHRPHE
4   PROPHEVALASPASPASNGLUVALSERSER
5   ALATHRPHEGLNSERLEUVALPROASPILE
6   PROGLYHISILEGLUSERPROVALPHEILE
7   ALATHRASN

Samples:

sample_1: Neh4ex, [U-13C; U-15N], 280 uM; MES 20 mM; DTT 1 mM

sample_2: Neh4ex, [U-15N], 300 uM; MES 20 mM; DTT 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
1H-15N heteronoesample_2isotropicsample_conditions_1
T1/R1 relaxationsample_2isotropicsample_conditions_1
T1rho/R1rho relaxationsample_2isotropicsample_conditions_1

Software:

NMRViewJ - chemical shift assignment

NMRDraw - Peak intensity measurements

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks