BMRB Entry 52418

Name: Backbone and aliphatic side-chain chemical shift assignment for 2N4R Tau
Published: 2024-04-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52418

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and aliphatic side-chain chemical shift assignment for 2N4R Tau

Deposition date:

2024-04-20

Original release date:

2024-04-30

Authors:

Crha, Radek; Kozelekova, Aneta; Hofrova, Alena; Ilkovicova, Lucia; Gasparik, Norbert; Kaderavek, Pavel; Hritz, Jozef

Citation:

Citation: Crha, Radek; Kozelekova, Aneta; Hofrova, Alena; Ilkovicova, Lucia; Gasparik, Norbert; Kaderavek, Pavel; Hritz, Jozef. "Hiding in plain sight: Complex interaction patterns between Tau and 14-3-3z protein variants." Int. J. Biol. Macromol. 266, 130802-130802 (2024).
PubMed: 38492709

Assembly members:

Assembly members:
entity_1, polymer, 442 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMAEPRQEFEVMEDHAGTYG LGDRKDQGGYTMHQDQEGDT DAGLKESPLQTPTEDGSEEP GSETSDAKSTPTAEDVTAPL VDEGAPGKQAAAQPHTEIPE GTTAEEAGIGDTPSLEDEAA GHVTQARMVSKSKDGTGSDD KKAKGADGKTKIATPRGAAP PGQKGQANATRIPAKTPPAP KTPPSSGEPPKSGDRSGYSS PGSPGTPGSRSRTPSLPTPP TREPKKVAVVRTPPKSPSSA KSRLQTAPVPMPDLKNVKSK IGSTENLKHQPGGGKVQIIN KKLDLSNVQSKCGSKDNIKH VPGGGSVQIVYKPVDLSKVT SKCGSLGNIHHKPGGGQVEV KSEKLDFKDRVQSKIGSLDN ITHVPGGGNKKIETHKLTFR ENAKAKTDHGAEIVYKSPVV SGDTSPRHLSNVSSTGSIDM VDSPQLATLADEVSASLAKQ GL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	1750
15N chemical shifts	440
1H chemical shifts	2203

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	2N4R Tau	1

Entities:

Entity 1, 2N4R Tau 442 residues - Formula weight is not available

1

SER

MET

ALA

GLU

PRO

ARG

GLN

GLU

PHE

GLU

2

VAL

MET

GLU

ASP

HIS

ALA

GLY

THR

TYR

GLY

3

LEU

GLY

ASP

ARG

LYS

ASP

GLN

GLY

TYR

4

THR

MET

HIS

GLN

ASP

GLN

GLU

GLY

ASP

THR

5

ASP

ALA

GLY

LEU

LYS

GLU

SER

PRO

LEU

GLN

6

THR

PRO

THR

GLU

ASP

GLY

SER

GLU

PRO

7

GLY

SER

GLU

THR

SER

ASP

ALA

LYS

SER

THR

8

PRO

THR

ALA

GLU

ASP

VAL

THR

ALA

PRO

LEU

9

VAL

ASP

GLU

GLY

ALA

PRO

GLY

LYS

GLN

ALA

10

ALA

GLN

PRO

HIS

THR

GLU

ILE

PRO

GLU

11

GLY

THR

ALA

GLU

ALA

GLY

ILE

GLY

12

ASP

THR

PRO

SER

LEU

GLU

ASP

GLU

ALA

13

GLY

HIS

VAL

THR

GLN

ALA

ARG

MET

VAL

SER

14

LYS

SER

LYS

ASP

GLY

THR

GLY

SER

ASP

15

LYS

ALA

LYS

GLY

ALA

ASP

GLY

LYS

THR

16

LYS

ILE

ALA

THR

PRO

ARG

GLY

ALA

PRO

17

PRO

GLY

GLN

LYS

GLY

GLN

ALA

ASN

ALA

THR

18

ARG

ILE

PRO

ALA

LYS

THR

PRO

ALA

PRO

19

LYS

THR

PRO

SER

GLY

GLU

PRO

20

LYS

SER

GLY

ASP

ARG

SER

GLY

TYR

SER

21

PRO

GLY

SER

PRO

GLY

THR

PRO

GLY

SER

ARG

22

SER

ARG

THR

PRO

SER

LEU

PRO

THR

PRO

23

THR

ARG

GLU

PRO

LYS

VAL

ALA

VAL

24

ARG

THR

PRO

LYS

SER

PRO

SER

ALA

25

LYS

SER

ARG

LEU

GLN

THR

ALA

PRO

VAL

PRO

26

MET

PRO

ASP

LEU

LYS

ASN

VAL

LYS

SER

LYS

27

ILE

GLY

SER

THR

GLU

ASN

LEU

LYS

HIS

GLN

28

PRO

GLY

LYS

VAL

GLN

ILE

ASN

29

LYS

LEU

ASP

LEU

SER

ASN

VAL

GLN

SER

30

LYS

CYS

GLY

SER

LYS

ASP

ASN

ILE

LYS

HIS

31

VAL

PRO

GLY

SER

VAL

GLN

ILE

VAL

32

TYR

LYS

PRO

VAL

ASP

LEU

SER

LYS

VAL

THR

33

SER

LYS

CYS

GLY

SER

LEU

GLY

ASN

ILE

HIS

34

HIS

LYS

PRO

GLY

GLN

VAL

GLU

VAL

35

LYS

SER

GLU

LYS

LEU

ASP

PHE

LYS

ASP

ARG

36

VAL

GLN

SER

LYS

ILE

GLY

SER

LEU

ASP

ASN

37

ILE

THR

HIS

VAL

PRO

GLY

ASN

LYS

38

LYS

ILE

GLU

THR

HIS

LYS

LEU

THR

PHE

ARG

39

GLU

ASN

ALA

LYS

ALA

LYS

THR

ASP

HIS

GLY

40

ALA

GLU

ILE

VAL

TYR

LYS

SER

PRO

VAL

41

SER

GLY

ASP

THR

SER

PRO

ARG

HIS

LEU

SER

42

ASN

VAL

SER

THR

GLY

SER

ILE

ASP

MET

43

VAL

ASP

SER

PRO

GLN

LEU

ALA

THR

LEU

ALA

44

ASP

GLU

VAL

SER

ALA

SER

LEU

ALA

LYS

GLN

45

GLY

LEU

Samples:

sample_1: 2N4R Tau, [U-100% 13C; U-100% 15N], 0.45 mM; sodium phosphate 20 mM; sodium azide 3 mM; D2O, [U-100% 2H], 10%; TCEP 2 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.8; pressure: 1 atm; temperature: 293.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
5D CACONCACO	sample_1	isotropic	sample_conditions_1
5D HC(CC-TOCSY)CACON	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking

NMRPipe - processing

Cleaner3D - processing

Reduced - processing

NMR spectrometers:

Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks