BMRB Entry 52417

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52417

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Title:
B2_3
Deposition date:
2024-04-19
Original release date:
2024-06-26
Authors:
Bi, Yunchen; Wu, Yujie
Citation:

Citation: Liu, Yuxin; Wu, Yujie; Bi, Yunchen. "1H, 13C and 15N resonance assignments of a variable domain of shark new antigen receptor against hyaluronan synthase"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAERVEQTPTTTTKEAGESL TVNCVLKGSSCALGNTYWYF TKKGATKKASLSTGGRYSDT KNTASKSFSLRISDLRVEDS GTYHCEAYTCAGGTPWRHYI EGGGTILTVK

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts97
1H chemical shifts474

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1A type of single domain antibody1

Entities:

Entity 1, A type of single domain antibody 110 residues - Formula weight is not available

1   METALAGLUARGVALGLUGLNTHRPROTHR
2   THRTHRTHRLYSGLUALAGLYGLUSERLEU
3   THRVALASNCYSVALLEULYSGLYSERSER
4   CYSALALEUGLYASNTHRTYRTRPTYRPHE
5   THRLYSLYSGLYALATHRLYSLYSALASER
6   LEUSERTHRGLYGLYARGTYRSERASPTHR
7   LYSASNTHRALASERLYSSERPHESERLEU
8   ARGILESERASPLEUARGVALGLUASPSER
9   GLYTHRTYRHISCYSGLUALATYRTHRCYS
10   ALAGLYGLYTHRPROTRPARGHISTYRILE
11   GLUGLYGLYGLYTHRILELEUTHRVALLYS

Samples:

sample_1: A type of single domain antibody, [U-100% 13C; U-100% 15N], 0.5 mM; D2O, [U-100% 2H], 10%; H2O 90%; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 0.3 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks