BMRB Entry 52401

Title:
Tau Domain (272-441) Phosphorylated by GSK3 beta
Deposition date:
2024-04-15
Original release date:
2025-03-25
Authors:
El Hajjar, Lea; Boll, Emmanuelle; Cantrelle, Francois-Xavier; Smet-Nocca, Caroline
Citation:

Citation: El Hajjar, Lea; Boll, Emmanuelle; Cantrelle, Francois-Xavier; Bridot, Clarisse; Landrieu, Isabelle; Smet-Nocca, Caroline. "Effect of PHF-1 hyperphosphorylation on the seeding activity of C-terminal Tau fragments"  Sci. Rep. 15, 9975-9975 (2025).
PubMed: 40121258

Assembly members:

Assembly members:
entity_1, polymer, 170 residues, 18176 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data typeCount
13C chemical shifts468
15N chemical shifts161
1H chemical shifts313

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1R2Ct-P1

Entities:

Entity 1, R2Ct-P 170 residues - 18176 Da.

X are phosphorylated Ser (pSer)

1   GLYGLYLYSVALGLNILEILEASNLYSLYS
2   LEUASPLEUSERASNVALGLNSERLYSCYS
3   GLYSERLYSASPASNILELYSHISVALPRO
4   GLYGLYGLYSERVALGLNILEVALTYRLYS
5   PROVALASPLEUSERLYSVALTHRSERLYS
6   CYSGLYSERLEUGLYASNILEHISHISLYS
7   PROGLYGLYGLYGLNVALGLUVALLYSSER
8   GLULYSLEUASPPHELYSASPARGVALGLN
9   SERLYSILEGLYSERLEUASPASNILETHR
10   HISVALPROGLYGLYGLYASNLYSLYSILE
11   GLUTHRHISLYSLEUTHRPHEARGGLUASN
12   ALALYSALALYSTHRASPHISGLYALAGLU
13   ILEVALTYRLYSSEPPROVALVALSEPGLY
14   ASPTHRSEPPROARGHISLEUSERASNVAL
15   SERSERTHRGLYSERILEASPMETVALASP
16   SERPROGLNLEUALATHRLEUALAASPGLU
17   VALSERALASERLEUALALYSGLNGLYLEU

Samples:

sample_1: R2Ct-P, [U-100% 13C; U-100% 15N], 0.35 mM; NaPi 25 mM; NaCl 50 mM; EDTA 2.5 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.092 M; pH: 6.600; pressure: 1.000 atm; temperature: 293.000 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
206 (H[N[co[{CA|ca[C]}]]])sample_1isotropicsample_conditions_1
208 (H[N[ca[CO]]])sample_1isotropicsample_conditions_1
211 (hNcaNH)sample_1isotropicsample_conditions_1
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
10230 (CO[N])sample_1isotropicsample_conditions_1
intra-HCANsample_1isotropicsample_conditions_1
225 (H{[N]+[HA]})sample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis v2.5 - Spectrum analysis, Spectrum display

TOPSPIN v4.3.1 - acquisition

NMRDraw vany - Spectrum analysis, Spectrum display

NMRPipe vany - Spectrum processing

qMDD v2.6 - processing

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

UniProt P10636-8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks