BMRB Entry 52399

Name: BRCT1
Published: 2024-06-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52399

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

BRCT1

Deposition date:

2024-04-15

Original release date:

2024-06-19

Authors:

Vu, Duc Duy; Pelupessy, Philippe; Wang, Ziqing; Carlier, Ludovic; Bouvignies, Guillaume; Ferrage, Fabien

Citation:

Citation: Vu, Duc-Duy; Bonucci, Alessio; Breniere, Manon; Cisneros-Aguirre, Metztli; Pelupessy, Philippe; Wang, Ziqing; Carlier, Ludovic; Bouvignies, Guillaume; Cortes, Patricia; Aggarwal, Aneel; Blackledge, Martin; Gueroui, Zoher; Belle, Valerie; Stark, Jeremy; Modesti, Mauro; Ferrage, Fabien. "Multivalent interactions of the disordered regions of XLF and XRCC4 foster robust cellular NHEJ and drive the formation of ligation-boosting condensates in vitro" Nat. Struct. Mol. Biol. ., .-. (2024).

Assembly members:

Assembly members:
entity_1, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMAL-c5x

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SGSKISNIFEDVEFCVMSGT DSQPKPDLENRIAEFGGYIV QNPGPDTYCVIAGSENIRVK NIILSNKHDVVKPAWLLECF KTKSFVPWQPRFMIHMCPST KEHFAREYD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	309
15N chemical shifts	98
1H chemical shifts	99

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BRCT1	1

Entities:

Entity 1, BRCT1 109 residues - Formula weight is not available

1

SER

GLY

SER

LYS

ILE

SER

ASN

ILE

PHE

GLU

2

ASP

VAL

GLU

PHE

CYS

VAL

MET

SER

GLY

THR

3

ASP

SER

GLN

PRO

LYS

PRO

ASP

LEU

GLU

ASN

4

ARG

ILE

ALA

GLU

PHE

GLY

TYR

ILE

VAL

5

GLN

ASN

PRO

GLY

PRO

ASP

THR

TYR

CYS

VAL

6

ILE

ALA

GLY

SER

GLU

ASN

ILE

ARG

VAL

LYS

7

ASN

ILE

LEU

SER

ASN

LYS

HIS

ASP

VAL

8

VAL

LYS

PRO

ALA

TRP

LEU

GLU

CYS

PHE

9

LYS

THR

LYS

SER

PHE

VAL

PRO

TRP

GLN

PRO

10

ARG

PHE

MET

ILE

HIS

MET

CYS

PRO

SER

THR

11

LYS

GLU

HIS

PHE

ALA

ARG

GLU

TYR

ASP

Samples:

sample_1: BRCT1, [U-100% 13C; U-100% 15N], 400-500 uM; Bistris 6.5 20 mM; potassium chloride 150 mM; EDTA 1 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

TOPSPIN - collection

NMR spectrometers:

Bruker AVANCE NEO 800 MHz