BMRB Entry 52396

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52396

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Title:
PfMATE pH6.8
Deposition date:
2024-04-14
Original release date:
2024-07-22
Authors:
Takeuchi, Koh; Imai, Misaki; Shimada, Ichio
Citation:

Citation: Takeuchi, Koh; Ueda, Takumi; Imai, Misaki; Fujisaki, Miwa; Shimura, Mie; Tokunaga, Yuji; Kofuku, Yutaka; Shimada, Ichio. "Affinity-directed substrate/H+-antiport by a MATE transporter"  Structure 32, 1150-1164 (2024).
PubMed: 38815577

Assembly members:

Assembly members:
entity_1, polymer, 471 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2157   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSEKTTKGVQLLRGDPKKAI VRLSIPMMIGMSVQTLYNLA DGIWVSGLGPESLAAVGLFF PVFMGIIALAAGLGVGTSSA IARRIGARDKEGADNVAVHS LILSLILGVTITITMLPAID SLFRSMGAKGEAVELAIEYA RVLLAGAFIIVFNNVGNGIL RGEGDANRAMLAMVLGSGLN IVLDPIFIYTLGFGVVGAAY ATLLSMVVTSLFIAYWLFVK RDTYVDITLRDFSPSREILK DILRVGLPSSLSQLSMSIAM FFLNSVAITAGGENGVAVFT SAWRITMLGIVPILGMAAAT TSVTGAAYGERNVEKLETAY LYAIKIAFMIELAVVAFIML FAPQVAYLFTYSESAQVIKG DLISALRTLPVFLVLTPFGM MTSAMFQGIGEGEKSLILTI FRTLVMQVGFAYIFVHYTTL GLRGVWIGIVIGNMVAAIVG FLWGRMRISALKKTSATGGK RLEKLHHHHHH

Data sets:
Data typeCount
13C chemical shifts49
1H chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PfMate11

Entities:

Entity 1, PfMate1 471 residues - Formula weight is not available

1   METSERGLULYSTHRTHRLYSGLYVALGLN
2   LEULEUARGGLYASPPROLYSLYSALAILE
3   VALARGLEUSERILEPROMETMETILEGLY
4   METSERVALGLNTHRLEUTYRASNLEUALA
5   ASPGLYILETRPVALSERGLYLEUGLYPRO
6   GLUSERLEUALAALAVALGLYLEUPHEPHE
7   PROVALPHEMETGLYILEILEALALEUALA
8   ALAGLYLEUGLYVALGLYTHRSERSERALA
9   ILEALAARGARGILEGLYALAARGASPLYS
10   GLUGLYALAASPASNVALALAVALHISSER
11   LEUILELEUSERLEUILELEUGLYVALTHR
12   ILETHRILETHRMETLEUPROALAILEASP
13   SERLEUPHEARGSERMETGLYALALYSGLY
14   GLUALAVALGLULEUALAILEGLUTYRALA
15   ARGVALLEULEUALAGLYALAPHEILEILE
16   VALPHEASNASNVALGLYASNGLYILELEU
17   ARGGLYGLUGLYASPALAASNARGALAMET
18   LEUALAMETVALLEUGLYSERGLYLEUASN
19   ILEVALLEUASPPROILEPHEILETYRTHR
20   LEUGLYPHEGLYVALVALGLYALAALATYR
21   ALATHRLEULEUSERMETVALVALTHRSER
22   LEUPHEILEALATYRTRPLEUPHEVALLYS
23   ARGASPTHRTYRVALASPILETHRLEUARG
24   ASPPHESERPROSERARGGLUILELEULYS
25   ASPILELEUARGVALGLYLEUPROSERSER
26   LEUSERGLNLEUSERMETSERILEALAMET
27   PHEPHELEUASNSERVALALAILETHRALA
28   GLYGLYGLUASNGLYVALALAVALPHETHR
29   SERALATRPARGILETHRMETLEUGLYILE
30   VALPROILELEUGLYMETALAALAALATHR
31   THRSERVALTHRGLYALAALATYRGLYGLU
32   ARGASNVALGLULYSLEUGLUTHRALATYR
33   LEUTYRALAILELYSILEALAPHEMETILE
34   GLULEUALAVALVALALAPHEILEMETLEU
35   PHEALAPROGLNVALALATYRLEUPHETHR
36   TYRSERGLUSERALAGLNVALILELYSGLY
37   ASPLEUILESERALALEUARGTHRLEUPRO
38   VALPHELEUVALLEUTHRPROPHEGLYMET
39   METTHRSERALAMETPHEGLNGLYILEGLY
40   GLUGLYGLULYSSERLEUILELEUTHRILE
41   PHEARGTHRLEUVALMETGLNVALGLYPHE
42   ALATYRILEPHEVALHISTYRTHRTHRLEU
43   GLYLEUARGGLYVALTRPILEGLYILEVAL
44   ILEGLYASNMETVALALAALAILEVALGLY
45   PHELEUTRPGLYARGMETARGILESERALA
46   LEULYSLYSTHRSERALATHRGLYGLYLYS
47   ARGLEUGLULYSLEUHISHISHISHISHIS
48   HIS

Samples:

sample_1: PfMATE, [U-2H; 99% 1HD-Ile; 99% 1HE-Met], 35 uM; D2O, [U-100% 2H], 99.6%; sodium phosphate 10 mM; sodium chloride 100 mM; n-Dodecyl-b-d-maltoside >0.2%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

TOPSPIN - collection, processing

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 800 MHz

Related Database Links:

Uniprot Q8U2X0