BMRB Entry 5239
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5239
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Elantak, Latifa; Bornet, Olivier; Morelli, Xavier; Dolla, Alain; Guerlesquin, Francoise. "Letter to the Editor: Sequential NMR Assignment of the Ferri-cytochrome c3 from
Desulfovibrio vulgaris Hildenborough" J. Biomol. NMR 23, 69-70 (2002).
PubMed: 12061720
Assembly members:
cytochrome c3, polymer, 107 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.
Natural source: Common Name: D.v.H. Taxonomy ID: 881 Superkingdom: Bacteria Kingdom: not available Genus/species: Desulfovibrio vulgaris
Experimental source: Production method: recombinant technology Host organism: Desulfovibrio desulfuricans Vector: pKJ800
Entity Sequences (FASTA):
cytochrome c3: APKAPADGLKMEATKQPVVF
NHSTHKSVKCGDCHHPVNGK
EDYRKCGTAGCHDSMDKKDK
SAKGYYHVMHDKNTKFKSCV
GCHVEVAGADAAKKKDLTGC
KKSKCHE
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 108 |
| 1H chemical shifts | 452 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | dvh c3ox monomer | 1 |
| 2 | heme, I | 2 |
| 3 | heme, II | 2 |
| 4 | heme, III | 2 |
| 5 | heme, IV | 2 |
Entities:
Entity 1, dvh c3ox monomer 107 residues - Formula weight is not available
| 1 | ALA | PRO | LYS | ALA | PRO | ALA | ASP | GLY | LEU | LYS | ||||
| 2 | MET | GLU | ALA | THR | LYS | GLN | PRO | VAL | VAL | PHE | ||||
| 3 | ASN | HIS | SER | THR | HIS | LYS | SER | VAL | LYS | CYS | ||||
| 4 | GLY | ASP | CYS | HIS | HIS | PRO | VAL | ASN | GLY | LYS | ||||
| 5 | GLU | ASP | TYR | ARG | LYS | CYS | GLY | THR | ALA | GLY | ||||
| 6 | CYS | HIS | ASP | SER | MET | ASP | LYS | LYS | ASP | LYS | ||||
| 7 | SER | ALA | LYS | GLY | TYR | TYR | HIS | VAL | MET | HIS | ||||
| 8 | ASP | LYS | ASN | THR | LYS | PHE | LYS | SER | CYS | VAL | ||||
| 9 | GLY | CYS | HIS | VAL | GLU | VAL | ALA | GLY | ALA | ASP | ||||
| 10 | ALA | ALA | LYS | LYS | LYS | ASP | LEU | THR | GLY | CYS | ||||
| 11 | LYS | LYS | SER | LYS | CYS | HIS | GLU |
Entity 2, heme, I - C34 H32 Fe N4 O4 - 616.487 Da.
| 1 | HEM |
Samples:
sample_1: cytochrome c3, [U-15N], 1.2 mM
set_cond_1: ionic strength: 0.02 M; pH: 5.9 na; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H COSY | sample_1 | not available | set_cond_1 |
| 2D 1H-1H TOCSY | sample_1 | not available | set_cond_1 |
| 2D 1H-1H NOESY | sample_1 | not available | set_cond_1 |
| 2D 1H-15N HSQC | sample_1 | not available | set_cond_1 |
| 2D 1H-13C HSQC | sample_1 | not available | set_cond_1 |
| 3D HNHA | sample_1 | not available | set_cond_1 |
| 3D 1H-1H-15N NOESY | sample_1 | not available | set_cond_1 |
| 3D 1H-1H-15N TOCSY | sample_1 | not available | set_cond_1 |
| 3D 15N-1H-15N HSQC-NOESY-HSQC | sample_1 | not available | set_cond_1 |
Software:
FELIX v98.0 - spectra processing
NMR spectrometers:
- Bruker AvanceDRX 500 MHz
Related Database Links:
| BMRB | 4291 5625 6634 |
| PDB | |
| EMBL | CAA27847 |
| GenBank | AAS97641 ABM27239 |
| REF | YP_012381 YP_965666 |
| SWISS-PROT | P00131 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks