BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5239

Title: Sequential NMR assignment of the ferri-cytochrome c3 from Desulfovibrio vulgaris Hildenborough   PubMed: 12061720

Deposition date: 2001-12-19 Original release date: 2003-01-07

Authors: Elantak, Latifa; Bornet, Olivier; Morelli, Xavier; Dolla, Alain; Guerlesquin, Francoise

Citation: Elantak, Latifa; Bornet, Olivier; Morelli, Xavier; Dolla, Alain; Guerlesquin, Francoise. "Letter to the Editor: Sequential NMR Assignment of the Ferri-cytochrome c3 from Desulfovibrio vulgaris Hildenborough"  J. Biomol. NMR 23, 69-70 (2002).

Assembly members:
cytochrome c3, polymer, 107 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: D.v.H.   Taxonomy ID: 881   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Desulfovibrio vulgaris

Experimental source:   Production method: recombinant technology   Host organism: Desulfovibrio desulfuricans   Vector: pKJ800

Entity Sequences (FASTA):
cytochrome c3: APKAPADGLKMEATKQPVVF NHSTHKSVKCGDCHHPVNGK EDYRKCGTAGCHDSMDKKDK SAKGYYHVMHDKNTKFKSCV GCHVEVAGADAAKKKDLTGC KKSKCHE

Data sets:
Data typeCount
15N chemical shifts108
1H chemical shifts452

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dvh c3ox monomer1
2heme, I2
3heme, II2
4heme, III2
5heme, IV2

Entities:

Entity 1, dvh c3ox monomer 107 residues - Formula weight is not available

1   ALAPROLYSALAPROALAASPGLYLEULYS
2   METGLUALATHRLYSGLNPROVALVALPHE
3   ASNHISSERTHRHISLYSSERVALLYSCYS
4   GLYASPCYSHISHISPROVALASNGLYLYS
5   GLUASPTYRARGLYSCYSGLYTHRALAGLY
6   CYSHISASPSERMETASPLYSLYSASPLYS
7   SERALALYSGLYTYRTYRHISVALMETHIS
8   ASPLYSASNTHRLYSPHELYSSERCYSVAL
9   GLYCYSHISVALGLUVALALAGLYALAASP
10   ALAALALYSLYSLYSASPLEUTHRGLYCYS
11   LYSLYSSERLYSCYSHISGLU

Entity 2, heme, I - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: cytochrome c3, [U-15N], 1.2 mM

set_cond_1: ionic strength: 0.02 M; pH: 5.9 na; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1not availableset_cond_1
2D 1H-1H TOCSYsample_1not availableset_cond_1
2D 1H-1H NOESYsample_1not availableset_cond_1
2D 1H-15N HSQCsample_1not availableset_cond_1
2D 1H-13C HSQCsample_1not availableset_cond_1
3D HNHAsample_1not availableset_cond_1
3D 1H-1H-15N NOESYsample_1not availableset_cond_1
3D 1H-1H-15N TOCSYsample_1not availableset_cond_1
3D 15N-1H-15N HSQC-NOESY-HSQCsample_1not availableset_cond_1

Software:

FELIX v98.0 - spectra processing

NMR spectrometers:

  • Bruker AvanceDRX 500 MHz

Related Database Links:

BMRB 4291 5625 6634
PDB
EMBL CAA27847
GenBank AAS97641 ABM27239
REF YP_012381 YP_965666
SWISS-PROT P00131

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts