BMRB Entry 52389
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52389
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ferreon, Josephine; Ta, Hai Minh; Yun, Hyosuk; Choi, Kyoung-jae; Quan, My Diem; Tsoi, Phoebe; Kim, Choel; Lee, Chul-won; Ferreon, Allan Chris. "Stereospecific NANOG PEST Stabilization by Pin1" Biochemistry 63, 1067-1074 (2024).
PubMed: 38619104
Assembly members:
entity_1, polymer, 26 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET302
Entity Sequences (FASTA):
entity_1: ETVXPLPSSMDLLIQDXPDS
STXPKG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 51 |
| 15N chemical shifts | 21 |
| 1H chemical shifts | 21 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Phosphorylated N-terminal domain | 1 |
Entities:
Entity 1, Phosphorylated N-terminal domain 26 residues - Formula weight is not available
| 1 | GLU | THR | VAL | SEP | PRO | LEU | PRO | SER | SER | MET | ||||
| 2 | ASP | LEU | LEU | ILE | GLN | ASP | SEP | PRO | ASP | SER | ||||
| 3 | SER | THR | SEP | PRO | LYS | GLY |
Samples:
sample_1: Phosphorylated Nanog NTD, [U-99% 13C; U-99% 15N], 250 uM; Sodium Phosphate 10 mM
sample_conditions_1: ionic strength: 40 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks