BMRB Entry 52383

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52383

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Title:
1H, 13C, and 15N Resonance Assignment of the La Motif of Human La-related Protein 1
Deposition date:
2024-04-01
Original release date:
2024-05-10
Authors:
Smith, Benjamin; Silvers, Robert
Citation:

Citation: Smith, Benjamin; Silvers, Robert. "1H, 13C, and 15N resonance assignments of the La Motif of the human La-related protein 1"  Biomol. NMR Assign. 18, 111-118 (2024).
PubMed: 38691336

Assembly members:

Assembly members:
entity_1, polymer, 1096 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MATQVEPLLPGGATLLQAEE HGGLVRKKPPPAPEGKGEPG PNDVRGGEPDGSARRPRPPC AKPHKEGTGQQERESPRPLQ LPGAEGPAISDGEEGGGEPG AGGGAAGAAGAGRRDFVEAP PPKVNPWTKNALPPVLTTVN GQSPPEHSAPAKVVRAAVPK QRKGSKVGDFGDAINWPTPG EIAHKSVQPQSHKPQPTRKL PPKKDMKEQEKGEGSDSKES PKTKSDESGEEKNGDEDCQR GGQKKKGNKHKWVPLQIDMK PEVPREKLASRPTRPPEPRH IPANRGEIKGSESATYVPVA PPTPAWQPEIKPEPAWHDQD ETSSVKSDGAGGARASFRGR GRGRGRGRGRGRGGTRTHFD YQFGYRKFDGVEGPRTPKYM NNITYYFDNVSSTELYSVDQ ELLKDYIKRQIEYYFSVDNL ERDFFLRRKMDADGFLPITL IASFHRVQALTTDISLIFAA LKDSKVVEIVDEKVRRREEP EKWPLPPIVDYSQTDFSQLL NCPEFVPRQHYQKETESAPG SPRAVTPVPTKTEEVSNLKT LPKGLSASLPDLDSENWIEV KKRPRPSPARPKKSEESRFS HLTSLPQQLPSQQLMSKDQD EQEELDFLFDEEMEQMDGRK NTFTAWSDEESDYEIDDRDV NKILIVTQTPHYMRRHPGGD RTGNHTSRAKMSAELAKVIN DGLFYYEQDLWAEKFEPEYS QIKQEVENFKKVNMISREQF DTLTPEPPVDPNQEVPPGPP RFQQVPTDALANKLFGAPEP STIARSLPTTVPESPNYRNT RTPRTPRTPQLKDSSQTSRF YPVVKEGRTLDAKMPRKRKT RHSSNPPLESHVGWVMDSRE HRPRTASISSSPSEGTPTVG SYGCTPQSLPKFQHPSHELL KENGFTQHVYHKYRRRCLNE RKRLGIGQSQEMNTLFRFWS FFLRDHFNKKMYEEFKQLAL EDAKEGYRYGLECLFRYYSY GLEKKFRLDIFKDFQEETVK DYEAGQLYGLEKFWAFLKYS KAKNLDIDPKLQEYLGKFRR LEDFRVDPPMGEEGNHKRHS VVAGGGGGEGRKRCPSQSSS RPAAMISQPPTPPTGQPVRE DAKWTSQHSNTQTLGK

Data sets:
Data typeCount
13C chemical shifts452
15N chemical shifts97
1H chemical shifts731

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LARP11

Entities:

Entity 1, LARP1 1096 residues - Formula weight is not available

1   METALATHRGLNVALGLUPROLEULEUPRO
2   GLYGLYALATHRLEULEUGLNALAGLUGLU
3   HISGLYGLYLEUVALARGLYSLYSPROPRO
4   PROALAPROGLUGLYLYSGLYGLUPROGLY
5   PROASNASPVALARGGLYGLYGLUPROASP
6   GLYSERALAARGARGPROARGPROPROCYS
7   ALALYSPROHISLYSGLUGLYTHRGLYGLN
8   GLNGLUARGGLUSERPROARGPROLEUGLN
9   LEUPROGLYALAGLUGLYPROALAILESER
10   ASPGLYGLUGLUGLYGLYGLYGLUPROGLY
11   ALAGLYGLYGLYALAALAGLYALAALAGLY
12   ALAGLYARGARGASPPHEVALGLUALAPRO
13   PROPROLYSVALASNPROTRPTHRLYSASN
14   ALALEUPROPROVALLEUTHRTHRVALASN
15   GLYGLNSERPROPROGLUHISSERALAPRO
16   ALALYSVALVALARGALAALAVALPROLYS
17   GLNARGLYSGLYSERLYSVALGLYASPPHE
18   GLYASPALAILEASNTRPPROTHRPROGLY
19   GLUILEALAHISLYSSERVALGLNPROGLN
20   SERHISLYSPROGLNPROTHRARGLYSLEU
21   PROPROLYSLYSASPMETLYSGLUGLNGLU
22   LYSGLYGLUGLYSERASPSERLYSGLUSER
23   PROLYSTHRLYSSERASPGLUSERGLYGLU
24   GLULYSASNGLYASPGLUASPCYSGLNARG
25   GLYGLYGLNLYSLYSLYSGLYASNLYSHIS
26   LYSTRPVALPROLEUGLNILEASPMETLYS
27   PROGLUVALPROARGGLULYSLEUALASER
28   ARGPROTHRARGPROPROGLUPROARGHIS
29   ILEPROALAASNARGGLYGLUILELYSGLY
30   SERGLUSERALATHRTYRVALPROVALALA
31   PROPROTHRPROALATRPGLNPROGLUILE
32   LYSPROGLUPROALATRPHISASPGLNASP
33   GLUTHRSERSERVALLYSSERASPGLYALA
34   GLYGLYALAARGALASERPHEARGGLYARG
35   GLYARGGLYARGGLYARGGLYARGGLYARG
36   GLYARGGLYGLYTHRARGTHRHISPHEASP
37   TYRGLNPHEGLYTYRARGLYSPHEASPGLY
38   VALGLUGLYPROARGTHRPROLYSTYRMET
39   ASNASNILETHRTYRTYRPHEASPASNVAL
40   SERSERTHRGLULEUTYRSERVALASPGLN
41   GLULEULEULYSASPTYRILELYSARGGLN
42   ILEGLUTYRTYRPHESERVALASPASNLEU
43   GLUARGASPPHEPHELEUARGARGLYSMET
44   ASPALAASPGLYPHELEUPROILETHRLEU
45   ILEALASERPHEHISARGVALGLNALALEU
46   THRTHRASPILESERLEUILEPHEALAALA
47   LEULYSASPSERLYSVALVALGLUILEVAL
48   ASPGLULYSVALARGARGARGGLUGLUPRO
49   GLULYSTRPPROLEUPROPROILEVALASP
50   TYRSERGLNTHRASPPHESERGLNLEULEU
51   ASNCYSPROGLUPHEVALPROARGGLNHIS
52   TYRGLNLYSGLUTHRGLUSERALAPROGLY
53   SERPROARGALAVALTHRPROVALPROTHR
54   LYSTHRGLUGLUVALSERASNLEULYSTHR
55   LEUPROLYSGLYLEUSERALASERLEUPRO
56   ASPLEUASPSERGLUASNTRPILEGLUVAL
57   LYSLYSARGPROARGPROSERPROALAARG
58   PROLYSLYSSERGLUGLUSERARGPHESER
59   HISLEUTHRSERLEUPROGLNGLNLEUPRO
60   SERGLNGLNLEUMETSERLYSASPGLNASP
61   GLUGLNGLUGLULEUASPPHELEUPHEASP
62   GLUGLUMETGLUGLNMETASPGLYARGLYS
63   ASNTHRPHETHRALATRPSERASPGLUGLU
64   SERASPTYRGLUILEASPASPARGASPVAL
65   ASNLYSILELEUILEVALTHRGLNTHRPRO
66   HISTYRMETARGARGHISPROGLYGLYASP
67   ARGTHRGLYASNHISTHRSERARGALALYS
68   METSERALAGLULEUALALYSVALILEASN
69   ASPGLYLEUPHETYRTYRGLUGLNASPLEU
70   TRPALAGLULYSPHEGLUPROGLUTYRSER
71   GLNILELYSGLNGLUVALGLUASNPHELYS
72   LYSVALASNMETILESERARGGLUGLNPHE
73   ASPTHRLEUTHRPROGLUPROPROVALASP
74   PROASNGLNGLUVALPROPROGLYPROPRO
75   ARGPHEGLNGLNVALPROTHRASPALALEU
76   ALAASNLYSLEUPHEGLYALAPROGLUPRO
77   SERTHRILEALAARGSERLEUPROTHRTHR
78   VALPROGLUSERPROASNTYRARGASNTHR
79   ARGTHRPROARGTHRPROARGTHRPROGLN
80   LEULYSASPSERSERGLNTHRSERARGPHE
81   TYRPROVALVALLYSGLUGLYARGTHRLEU
82   ASPALALYSMETPROARGLYSARGLYSTHR
83   ARGHISSERSERASNPROPROLEUGLUSER
84   HISVALGLYTRPVALMETASPSERARGGLU
85   HISARGPROARGTHRALASERILESERSER
86   SERPROSERGLUGLYTHRPROTHRVALGLY
87   SERTYRGLYCYSTHRPROGLNSERLEUPRO
88   LYSPHEGLNHISPROSERHISGLULEULEU
89   LYSGLUASNGLYPHETHRGLNHISVALTYR
90   HISLYSTYRARGARGARGCYSLEUASNGLU
91   ARGLYSARGLEUGLYILEGLYGLNSERGLN
92   GLUMETASNTHRLEUPHEARGPHETRPSER
93   PHEPHELEUARGASPHISPHEASNLYSLYS
94   METTYRGLUGLUPHELYSGLNLEUALALEU
95   GLUASPALALYSGLUGLYTYRARGTYRGLY
96   LEUGLUCYSLEUPHEARGTYRTYRSERTYR
97   GLYLEUGLULYSLYSPHEARGLEUASPILE
98   PHELYSASPPHEGLNGLUGLUTHRVALLYS
99   ASPTYRGLUALAGLYGLNLEUTYRGLYLEU
100   GLULYSPHETRPALAPHELEULYSTYRSER
101   LYSALALYSASNLEUASPILEASPPROLYS
102   LEUGLNGLUTYRLEUGLYLYSPHEARGARG
103   LEUGLUASPPHEARGVALASPPROPROMET
104   GLYGLUGLUGLYASNHISLYSARGHISSER
105   VALVALALAGLYGLYGLYGLYGLYGLUGLY
106   ARGLYSARGCYSPROSERGLNSERSERSER
107   ARGPROALAALAMETILESERGLNPROPRO
108   THRPROPROTHRGLYGLNPROVALARGGLU
109   ASPALALYSTRPTHRSERGLNHISSERASN
110   THRGLNTHRLEUGLYLYS

Samples:

sample_1: LARP1, [U-98% 13C; U-98% 15N], 1.3 mM; TRIS/HCl 20 mM; sodium chloride 100 mM; DSS 0.01 % w/v

sample_2: LARP1, [U-65% 2H; U-98% 13C; U-98% 15N], 900 uM; TRIS/HCl 20 mM; sodium chloride 100 mM; DSS 0.01 % w/v

sample_3: LARP1, [U-15N]-Ile, 380 uM; TRIS/HCl 20 mM; sodium chloride 100 mM; DSS 0.01 % w/v

sample_4: LARP1, [U-15N]-Val, 480 uM; TRIS/HCl 20 mM; sodium chloride 100 mM; DSS 0.01 % w/v

sample_5: LARP1, [U-15N]-Leu, 420 uM; TRIS/HCl 20 mM; sodium chloride 100 mM; DSS 0.01 % w/v

sample_6: LARP1, [U-15N]-Phe, 340 uM; TRIS/HCl 20 mM; sodium chloride 100 mM; DSS 0.01 % w/v

sample_7: LARP1, [U-13C,U-15N]-Arg, 230 uM; TRIS/HCl 20 mM; sodium chloride 100 mM; DSS 0.01 % w/v

sample_8: LARP1, [2-13C-glycerol,U-15N], 640 uM; TRIS/HCl 20 mM; sodium chloride 100 mM; DSS 0.01 % w/v

sample_9: LARP1, [1,3-13C-glycerol,U-15N], 730 uM; TRIS/HCl 20 mM; sodium chloride 100 mM; DSS 0.01 % w/v

sample_conditions_1: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_5isotropicsample_conditions_1
2D 1H-15N HSQCsample_6isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_6isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_7isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_7isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_8isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_8isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_9isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_7isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_7isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_8isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_8isotropicsample_conditions_1

Software:

TOPSPIN3.6.0 v3.6.0 - collection

TOPSPIN4.2.0 v4.2.0 - processing

NMRFAM-SPARKY v4.2.0 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Related Database Links:

UNP Q6PKG0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks