BMRB Entry 52376
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52376
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Citation: Adhada, Sri Teja; Sarma, Siddhartha. "Slow conformational exchange between partially folded and near native states of Ubiquitin suggest a multi-state folding model" .
Assembly members:
entity_1, polymer, 77 residues, Formula weight is not available
Natural source: Common Name: Japanese rice Taxonomy ID: 39947 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Oryza sativa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a(+)
Entity Sequences (FASTA):
entity_1: MMQIFVKTLTGKTITLEVES
SDTIDNVKAKIQDKEGIPPD
QQRLIFAGKQLEDGRTLADY
NIQKESTLHLVLRLRGG'
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 324 |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 74 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ubiquitin folding intermediate | 1 |
Entities:
Entity 1, Ubiquitin folding intermediate 77 residues - Formula weight is not available
| 1 | MET | MET | GLN | ILE | PHE | VAL | LYS | THR | LEU | THR | ||||
| 2 | GLY | LYS | THR | ILE | THR | LEU | GLU | VAL | GLU | SER | ||||
| 3 | SER | ASP | THR | ILE | ASP | ASN | VAL | LYS | ALA | LYS | ||||
| 4 | ILE | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ASP | ||||
| 5 | GLN | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | GLN | ||||
| 6 | LEU | GLU | ASP | GLY | ARG | THR | LEU | ALA | ASP | TYR | ||||
| 7 | ASN | ILE | GLN | LYS | GLU | SER | THR | LEU | HIS | LEU | ||||
| 8 | VAL | LEU | ARG | LEU | ARG | GLY | GLY |
Samples:
sample_1: Ubiquitin from Rice (Oryza sativa) 1 ± 0.1 mM
sample_2: Ubiquitin from Rice (Oryza sativa), [U-15N], 1 ± 0.1 mM
sample_3: Ubiquitin from Rice (Oryza sativa), [U-13C; U-15N], 1 ± 0.1 mM
sample_conditions_1: ionic strength: 20 mM; pH: 2; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_3 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_3 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_3 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_3 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_3 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_3 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.5.2 - chemical shift assignment, peak picking
NMRPipe v10.9 - data analysis, processing
VNMRj v4.2 - collection
TOPSPIN v4.0.9 - collection
NMR spectrometers:
- Bruker Avance 700 MHz
- Agilent DirectDrive 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks