BMRB Entry 52375

Title:
Casein Kinase 1 delta 2 tail
Deposition date:
2024-03-27
Original release date:
2024-08-28
Authors:
Harold, Rachel; Lee, Hsiau-Wei; Partch, Carrie
Citation:

Citation: Harold, Rachel; Tulsian, Nikhil; Narasimamurthy, Rajesh; Yaitanes, Noelle; Ayala Hernandez, Maria; Lee, Hsiau-Wei; Crosby, Priya; Tripathi, Sarvind; Virshup, David; Partch, Carrie. "Isoform-specific C-terminal phosphorylation drives autoinhibition of Casein Kinase 1"  bioRxiv ., .-. (2024).
PubMed: 39131317

Assembly members:

Assembly members:
entity_1, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet22b

Data sets:
Data typeCount
13C chemical shifts6
15N chemical shifts6
1H chemical shifts5

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CK1d tail1

Entities:

Entity 1, CK1d tail 123 residues - Formula weight is not available

1   GLYALAMETASPPROGLUPHETRPPHEGLY
2   ALASERARGALAALAASPASPALAGLUARG
3   GLUARGARGASPARGGLUGLUARGLEUARG
4   HISSERARGASNPROALATHRARGGLYLEU
5   PROSERTHRALASERGLYARGLEUARGGLY
6   THRGLNGLUVALALAPROPROTHRPROLEU
7   THRPROTHRSERHISTHRALAASNTHRSER
8   PROARGPROVALSERGLYMETGLUARGGLU
9   ARGLYSVALSERMETARGLEUHISARGGLY
10   ALAPROVALASNILESERSERSERASPLEU
11   THRGLYARGGLNASPTHRSERARGMETSER
12   THRSERGLNASNSERILEPROPHEGLUHIS
13   HISGLYLYS

Samples:

sample_1: CK1d2 tail, [U-100% 13C; U-100% 15N], 500 uM; D2O 10%; EDTA 1 mM; sodium chloride 100 mM; beta-mercaptoethanol 5 mM; mops 25 mM; tween 0.05%; Magnesium chloride 11 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 13C-15N CONsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HACANCACONsample_1isotropicsample_conditions_1
3D HACANCANCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMRPipe - processing

POKY - chemical shift assignment

NMRDraw - processing

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks